Difference between revisions of "Part:BBa K4225008:Design"
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===Design Notes=== | ===Design Notes=== | ||
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===Source=== | ===Source=== | ||
| − | + | All parts except TEV protease are obtained from biobricks. For the source of TEV protease, refer to the basic part BBa_K4225009. Prefix-Suffix are added to our sequence and spacers are also added between the parts. This composite part is also codon optimized for our chassis, DH5α. | |
===References=== | ===References=== | ||
Latest revision as of 14:37, 12 October 2022
TEV protease induced by Ptac and cMyc-tag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 855
Illegal XhoI site found at 165 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 439
Illegal SapI.rc site found at 787
Design Notes
Source
All parts except TEV protease are obtained from biobricks. For the source of TEV protease, refer to the basic part BBa_K4225009. Prefix-Suffix are added to our sequence and spacers are also added between the parts. This composite part is also codon optimized for our chassis, DH5α.