Difference between revisions of "Part:BBa K174000:Design"
(New page: 498 bp long sspB CDS is amplified by PCR with dangling end primers with EcoRI-NotI-XbaI restriction site at 5’ and SpeI at 3’ and inserted into a Biobrick compatible vector. The sequen...) |
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− | + | The sequence is taken from E. coli strain DH5 alpha. Genbank accession number for E. coli MG1655 strain is NC_000913.2 | |
− | + | ==Construction== | |
− | + | Plasmid part BBa_J04450 (pSB1AT3) and our construct were treated with EcoRI and SpeI | |
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[[Image:Newcastle_2009_sspB_1.png]] | [[Image:Newcastle_2009_sspB_1.png]] | ||
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+ | The backbone and the sspB insert were then ligated | ||
[[Image:Newcastle_2009_sspB_2.png]] | [[Image:Newcastle_2009_sspB_2.png]] |
Latest revision as of 22:36, 21 October 2009
The sequence is taken from E. coli strain DH5 alpha. Genbank accession number for E. coli MG1655 strain is NC_000913.2
Construction
Plasmid part BBa_J04450 (pSB1AT3) and our construct were treated with EcoRI and SpeI
The backbone and the sspB insert were then ligated