Difference between revisions of "Part:BBa K4343082"

 
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<partinfo>BBa_K4343082 short</partinfo>
 
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IgASE1 gene encodes &#8710;-9 elongase from Isochrysis galbana. This enzyme can catalyze the elongation of C18:2 to C20:2 in Y. lipolytica.
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IgASE1 gene encodes ∆-9 elongase from Isochrysis galbana. This enzyme can catalyze the elongation of C18:2 to C20:2 in Y. lipolytica.
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===Characterisation===
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We integrated IgASE1 controlled by the TEF promoter to the SCP2 site of the genome of Y. lipolytica through this segment. Then we detected the proportion of C18:2 and C20:2 by gas chromatography (GC) and test the activity of ∆-9 elongase according to the results.<br>
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<center>https://static.igem.wiki/teams/4343/wiki/puc-huh-scp2-igase1-map.png</center>
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===Result===
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The conversion of C18:2 to C20:2 under the catalytic action of IgASE1 was around 26.3%, with only 16.5% of C20:2 produced. (Fig: Po1f-9)<br>
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<center>https://static.igem.wiki/teams/4343/wiki/part-6.jpg</center>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 12:02, 9 October 2022


pUC-HUH-Scp2-IgASE1

IgASE1 gene encodes ∆-9 elongase from Isochrysis galbana. This enzyme can catalyze the elongation of C18:2 to C20:2 in Y. lipolytica.

Characterisation

We integrated IgASE1 controlled by the TEF promoter to the SCP2 site of the genome of Y. lipolytica through this segment. Then we detected the proportion of C18:2 and C20:2 by gas chromatography (GC) and test the activity of ∆-9 elongase according to the results.

puc-huh-scp2-igase1-map.png

Result

The conversion of C18:2 to C20:2 under the catalytic action of IgASE1 was around 26.3%, with only 16.5% of C20:2 produced. (Fig: Po1f-9)

part-6.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1431
    Illegal XhoI site found at 3465
    Illegal XhoI site found at 3498
    Illegal XhoI site found at 7817
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3653
    Illegal AgeI site found at 889
    Illegal AgeI site found at 1543
    Illegal AgeI site found at 1642
    Illegal AgeI site found at 2003
    Illegal AgeI site found at 4706
    Illegal AgeI site found at 5067
    Illegal AgeI site found at 6209
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI site found at 1546
    Illegal BsaI site found at 2183
    Illegal BsaI site found at 5247
    Illegal BsaI site found at 5784
    Illegal BsaI site found at 6212
    Illegal BsaI site found at 7026
    Illegal BsaI.rc site found at 401
    Illegal BsaI.rc site found at 1540
    Illegal BsaI.rc site found at 6206
    Illegal BsaI.rc site found at 7020
    Illegal BsaI.rc site found at 7290
    Illegal BsaI.rc site found at 8452
    Illegal BsaI.rc site found at 8836
    Illegal SapI site found at 6501
    Illegal SapI.rc site found at 1908
    Illegal SapI.rc site found at 4972
    Illegal SapI.rc site found at 6984