Difference between revisions of "Part:BBa K4343080"
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− | BgElo9 was thought to have no preference towards C18:2. | + | BgElo9 was thought to have no preference towards C18:2. (Fig: Po1f-7)<br> |
+ | <center>https://static.igem.wiki/teams/4343/wiki/part-6.jpg</center> | ||
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− | <span class='h3bb'> | + | <span class='h3bb'>Sequence and Features</span> |
<partinfo>BBa_K4343080 SequenceAndFeatures</partinfo> | <partinfo>BBa_K4343080 SequenceAndFeatures</partinfo> | ||
Latest revision as of 12:01, 9 October 2022
pUC-HUH-Scp2-BgElo9
BgElo9 gene encodes ∆-9 elongase from Blattella germanica. This enzyme can catalyze the elongation of C18:2 to C20:2 in Y. lipolytica.
Characterisation
We integrated BgElo9 controlled by the TEF promoter to the SCP2 site of the genome of Y. lipolytica through this segment. Then we detected the proportion of C18:2 and C20:2 by gas chromatography (GC) and test the activity of ∆-9 elongase according to the results.
![puc-huh-scp2-bgelo9-map.png](https://static.igem.wiki/teams/4343/wiki/puc-huh-scp2-bgelo9-map.png)
Result
BgElo9 was thought to have no preference towards C18:2. (Fig: Po1f-7)
![part-6.jpg](https://static.igem.wiki/teams/4343/wiki/part-6.jpg)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 7015
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1431
Illegal XhoI site found at 3465
Illegal XhoI site found at 3498
Illegal XhoI site found at 8012 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 3653
Illegal AgeI site found at 889
Illegal AgeI site found at 1543
Illegal AgeI site found at 1642
Illegal AgeI site found at 2003
Illegal AgeI site found at 4706
Illegal AgeI site found at 5067
Illegal AgeI site found at 6209 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1
Illegal BsaI site found at 1546
Illegal BsaI site found at 2183
Illegal BsaI site found at 5247
Illegal BsaI site found at 5784
Illegal BsaI site found at 6212
Illegal BsaI site found at 6739
Illegal BsaI.rc site found at 401
Illegal BsaI.rc site found at 1540
Illegal BsaI.rc site found at 6206
Illegal BsaI.rc site found at 6268
Illegal BsaI.rc site found at 7215
Illegal BsaI.rc site found at 7485
Illegal BsaI.rc site found at 8647
Illegal SapI.rc site found at 1908
Illegal SapI.rc site found at 4972