Difference between revisions of "Part:BBa K4156087"

 
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===Usage and Biology===
 
===Usage and Biology===
  
As one of the key players in the genetic switch, it catalyzes the unidirectional inverse reaction according to the position and orientation of its specific recognition sites, attB and attP. We introduce this element to control the switching of genetic logic gates. It also acts as a genetic signal amplifier
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As one of the key players in the genetic switch, it catalyzes the unidirectional inverse reaction according to the position and orientation of its specific recognition sites, attB and attP. <sup>[1]</sup> We introduce this element to control the switching of genetic logic gates. It also acts as a genetic signal amplifier
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===Characterization===
  
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We introduce this element to control the switching of genetic logic gates. Characterization details can be found at<html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/Part:BBa_K415699"> BBa_K415699 </a></html> ; <html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/Part:BBa_K4156100"> BBa_K4156100 </a></html> (pCadC Promoter); <html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/Part:BBa_K4156104"> BBa_K4156104 </a></html> ; <html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/Part:BBa_K4156105"> BBa_K4156105 </a></html> (pLldR Promoter) ; <html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/Part:BBa_K4156108"> BBa_K4156108 </a></html> ; <html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/Part:BBa_K4156109"> BBa_K4156109 </a></html> (pPepT Promoter) ; <html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/Part:BBa_K4156117"> BBa_K4156117 </a></html> ; <html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/Part:BBa_K4156118"> BBa_K4156118 </a></html> ;<html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/Part:BBa_K4156119"> BBa_K4156119 </a></html>(XOR gate).
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===References===
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<i>
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1 Courbet A, Endy D, Renard E, Molina F, Bonnet J. Detection of pathological biomarkers in human clinical samples via amplifying genetic switches and logic gates. Sci Transl Med. May 27 2015;7(289):289ra83. doi:10.1126/scitranslmed.aaa3601
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</i>
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K4156087 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4156087 SequenceAndFeatures</partinfo>

Latest revision as of 11:57, 11 October 2022


Integrase TP901

TP901 is a kind of Serine recombinase.


Usage and Biology

As one of the key players in the genetic switch, it catalyzes the unidirectional inverse reaction according to the position and orientation of its specific recognition sites, attB and attP. [1] We introduce this element to control the switching of genetic logic gates. It also acts as a genetic signal amplifier

Characterization

We introduce this element to control the switching of genetic logic gates. Characterization details can be found at BBa_K415699  ; BBa_K4156100 (pCadC Promoter); BBa_K4156104  ; BBa_K4156105 (pLldR Promoter) ; BBa_K4156108  ; BBa_K4156109 (pPepT Promoter) ; BBa_K4156117  ; BBa_K4156118  ; BBa_K4156119 (XOR gate).

References

1 Courbet A, Endy D, Renard E, Molina F, Bonnet J. Detection of pathological biomarkers in human clinical samples via amplifying genetic switches and logic gates. Sci Transl Med. May 27 2015;7(289):289ra83. doi:10.1126/scitranslmed.aaa3601

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1456
  • 1000
    COMPATIBLE WITH RFC[1000]