Difference between revisions of "Part:BBa K4156088"

Latest revision as of 11:59, 11 October 2022

Integrase Bxb1

Bxb1 integrase serves as a highly efficient DNA recombinase

Usage and Biology

The mycobacteriophage large serine recombinase Bxb1 catalyzes site-specific recombination between its corresponding attP and attB recognition sites.^[1] We introduce this element to control the switching of genetic logic gates It also acts as a genetic signal amplifier.

References

1 Courbet A, Endy D, Renard E, Molina F, Bonnet J. Detection of pathological biomarkers in human clinical samples via amplifying genetic switches and logic gates. Sci Transl Med. May 27 2015;7(289):289ra83. doi:10.1126/scitranslmed.aaa3601

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 192
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 466
Illegal XhoI site found at 553
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1300

@@ Line 9: / Line 9: @@
 ===Usage and Biology===
-The mycobacteriophage large serine recombinase Bxb1 catalyzes site-specific recombination between its corresponding attP and attB recognition sites. We introduce this element to control the switching of genetic logic gates It also acts as a genetic signal amplifier.
+The mycobacteriophage large serine recombinase Bxb1 catalyzes site-specific recombination between its corresponding attP and attB recognition sites.<sup>[1]</sup> We introduce this element to control the switching of genetic logic gates It also acts as a genetic signal amplifier.
+===References===
+<i>
+Courbet A, Endy D, Renard E, Molina F, Bonnet J. Detection of pathological biomarkers in human clinical samples via amplifying genetic switches and logic gates. Sci Transl Med. May 27 2015;7(289):289ra83. doi:10.1126/scitranslmed.aaa3601
+</i>
 <span class='h3bb'>Sequence and Features</span>