Difference between revisions of "Part:BBa K4414005"
 
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<partinfo>BBa_K4414005 short</partinfo>
 
<partinfo>BBa_K4414005 short</partinfo>
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==Usage and Biology==
 
==Usage and Biology==
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There is an extensive use of fluorescent bioimaging in the fields of biochemistry, biotechnology, cell and developmental biology. In comparison with other fluorescent tags, GFP-like proteins have the advantage of forming internal chromophore without requiring accessory cofactors, enzymes or substrates other than molecular oxygen, making possible chromophore formation in live organisms, tissues and cells(Stepanenko et al., 2008;Cubitt et al., 1995). We obtained this DNA by DNA synthesis.This part can be used in level 1 biological laboratory.
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There is an extensive use of fluorescent bioimaging in the fields of biochemistry, biotechnology, cell and developmental biology. In comparison with other fluorescent tags, GFP-like proteins have the advantage of forming internal chromophore without requiring accessory cofactors, enzymes or substrates other than molecular oxygen, making possible chromophore formation in live organisms, tissues and cells.(Stepanenko et al., 2008)(Cubitt et al., 1995)
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===Sequecing===
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The plasmid was sequenced correct.
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<figure class="figure">
 
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<img src="https://static.igem.wiki/teams/4414/wiki/05-1.png" class="figure-img img-fluid rounded"  height="200px">
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<img src="https://static.igem.wiki/teams/4414/wiki/05-1.png" class="figure-img img-fluid rounded"  height="400px">
  
 
</figure>
 
</figure>
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Figure 1.The picture of fluorescence diagram
  
 
===Reference===
 
===Reference===
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[1].Cubitt, A. B., Heim, R., Adams, S. R., Boyd, A. E., Gross, L. A., & Tsien, R. Y. (1995). Understanding, improving and using green fluorescent proteins. Trends in Biochemical Sciences, 20(11), 448–455. https://doi.org/10.1016/s0968-0004(00)89099-4
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1.Cubitt, A. B., Heim, R., Adams, S. R., Boyd, A. E., Gross, L. A., & Tsien, R. Y. (1995). Understanding, improving and using green fluorescent proteins. Trends in Biochemical Sciences, 20(11), 448–455. https://doi.org/10.1016/s0968-0004(00)89099-4.
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[2].Stepanenko, O. V., Verkhusha, V. V., Kuznetsova, I. M., Uversky, V. N., & Turoverov, K. K. (2008). Fluorescent Proteins as Biomarkers and Biosensors: Throwing Color Lights on Molecular and Cellular Processes. Current Protein & Peptide Science, 9(4), 338–369.
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2.Stepanenko, O. V., Verkhusha, V. V., Kuznetsova, I. M., Uversky, V. N., & Turoverov, K. K. (2008). Fluorescent Proteins as Biomarkers and Biosensors: Throwing Color Lights on Molecular and Cellular Processes. Current Protein & Peptide Science, 9(4), 338–369.

Latest revision as of 03:49, 14 October 2022


GFP

Usage and Biology

There is an extensive use of fluorescent bioimaging in the fields of biochemistry, biotechnology, cell and developmental biology. In comparison with other fluorescent tags, GFP-like proteins have the advantage of forming internal chromophore without requiring accessory cofactors, enzymes or substrates other than molecular oxygen, making possible chromophore formation in live organisms, tissues and cells(Stepanenko et al., 2008;Cubitt et al., 1995). We obtained this DNA by DNA synthesis.This part can be used in level 1 biological laboratory.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Functional characterization

We connect GFP as a reporter gene to other sequences encoding proteins, as shown in the figure is the fluorescence image of GFP.

Figure 1.The picture of fluorescence diagram

Reference

1.Cubitt, A. B., Heim, R., Adams, S. R., Boyd, A. E., Gross, L. A., & Tsien, R. Y. (1995). Understanding, improving and using green fluorescent proteins. Trends in Biochemical Sciences, 20(11), 448–455. https://doi.org/10.1016/s0968-0004(00)89099-4.

2.Stepanenko, O. V., Verkhusha, V. V., Kuznetsova, I. M., Uversky, V. N., & Turoverov, K. K. (2008). Fluorescent Proteins as Biomarkers and Biosensors: Throwing Color Lights on Molecular and Cellular Processes. Current Protein & Peptide Science, 9(4), 338–369.