Difference between revisions of "Part:BBa K4343104"

 
 
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<partinfo>BBa_K4343104 short</partinfo>
 
<partinfo>BBa_K4343104 short</partinfo>
  
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IgASE1 encodes &#8710;-9 elongase from Isochrysis galbana which functions in the &#916;-9 elongase/desaturase pathway and could convert C18:2 to C20:2.
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IgASE1 encodes ∆-9 elongase from Isochrysis galbana which functions in the Δ-9 elongase/desaturase pathway and could convert C18:2 to C20:2.
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====Characterization====
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Heterologous expression of IgASE1 could enable engineered Y. lipolytica to produce C20:2 fatty acids that couldn’t be produced in wild-type Y. lipolytica.
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====Literature====
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Baoxiu Qi characterized the function, activity and substrate specificity of IgASE1 in Saccharomyces cerevisiae. The engineered yeast cells could specifically convert the C18-∆9 polyunsaturated fatty acids, linoleic acid (C18:2n-6, ∆9;12) and α-linolenic acid (C18:3n-3, ∆9;12;15), to eicosadienoic acid (C20:2n-6, ∆11;14) and eicosatrienoic acid (C20:3n-3, ∆11;14;17), respectively.
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====Reference====
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Qi, B., Beaudoin, F., Fraser, T., Stobart, A. K., Napier, J. A., & Lazarus, C. M. (2002). Identification of a cDNA encoding a novel C18-Δ9 polyunsaturated fatty acid-specific elongating activity from the docosahexaenoic acid (DHA)-producing microalga, Isochrysis galbana. FEBS letters, 510(3), 159-165.
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===Usage and Biology===
 
  
 
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Latest revision as of 08:26, 9 October 2022


IgASE1

IgASE1 encodes ∆-9 elongase from Isochrysis galbana which functions in the Δ-9 elongase/desaturase pathway and could convert C18:2 to C20:2.

Characterization

Heterologous expression of IgASE1 could enable engineered Y. lipolytica to produce C20:2 fatty acids that couldn’t be produced in wild-type Y. lipolytica.

Literature

Baoxiu Qi characterized the function, activity and substrate specificity of IgASE1 in Saccharomyces cerevisiae. The engineered yeast cells could specifically convert the C18-∆9 polyunsaturated fatty acids, linoleic acid (C18:2n-6, ∆9;12) and α-linolenic acid (C18:3n-3, ∆9;12;15), to eicosadienoic acid (C20:2n-6, ∆11;14) and eicosatrienoic acid (C20:3n-3, ∆11;14;17), respectively.

Reference

Qi, B., Beaudoin, F., Fraser, T., Stobart, A. K., Napier, J. A., & Lazarus, C. M. (2002). Identification of a cDNA encoding a novel C18-Δ9 polyunsaturated fatty acid-specific elongating activity from the docosahexaenoic acid (DHA)-producing microalga, Isochrysis galbana. FEBS letters, 510(3), 159-165.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 246
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 809
    Illegal SapI site found at 290
    Illegal SapI.rc site found at 773