Difference between revisions of "Part:BBa K4137006"
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===Construct Designs=== | ===Construct Designs=== | ||
| − | We attached a 6x His-Tag downstream of the ccdA sequence | + | We attached a 6x His-Tag downstream of the ccdA sequence to determine the basal production of the antitoxin. A p_mleS promoter, which induces protein production when malate is present, as well as a 5'UTR RBS (AAGGAG), are attached upstream to the side of the open reading frame (ccdA-6x his tag). The terminator BBa_B1006 is attached downstream of the sequence. |
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===Sequence and Features=== | ===Sequence and Features=== | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Latest revision as of 07:52, 10 October 2022
CcdA expressing construct
This sequence codes for CcdA, the antitoxin which assures chi18h8’s production around the plant roots. The mleR regulatory site comes from p_mles, which binds with the RBS and CcdA, the antitoxin that will bind with CcdB when induced by malate acid. This will detoxify ccdB. The purification tag 6xHis, and the terminator B1006 (BBa_B1006) are flanked on the end of the open reading frame.
Construct Designs
We attached a 6x His-Tag downstream of the ccdA sequence to determine the basal production of the antitoxin. A p_mleS promoter, which induces protein production when malate is present, as well as a 5'UTR RBS (AAGGAG), are attached upstream to the side of the open reading frame (ccdA-6x his tag). The terminator BBa_B1006 is attached downstream of the sequence.
Sequence and Features
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]