Difference between revisions of "Part:BBa K4156103"
 
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<partinfo>BBa_K4156103 short</partinfo>
 
<partinfo>BBa_K4156103 short</partinfo>
  
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pLldR-Bxb1 is constructed with L-lactate-sensitive promoter pLldR and serine integrase Bxb1, and is uesd for lactate-specific response for logic gate coupling.
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pLldR-Bxb1-φ174E is a biobrick that expresses the cleavage gene φ174E by pLldR-Bxb1 co-control.
  
  
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===Usage and Biology===
 
===Usage and Biology===
  
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pLldR-Bxb1 consists of a fusion of the lactate-sensitive promoter pLldR and the serine integrase Bxb1. It will act as a complex regulatory for controlling downstream logic gates and transcription of genes.
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We designed pLldR-Bxb1-φ174E to test the expression efficiency of φ174E under the control of a logic gate linking the pLldR and Bxb1. We will validate the function of this biobrick by measuring the viability of the bacteria.
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Latest revision as of 13:22, 11 October 2022


pLldR-Bxb1-φ174E

pLldR-Bxb1-φ174E is a biobrick that expresses the cleavage gene φ174E by pLldR-Bxb1 co-control.


Usage and Biology

We designed pLldR-Bxb1-φ174E to test the expression efficiency of φ174E under the control of a logic gate linking the pLldR and Bxb1. We will validate the function of this biobrick by measuring the viability of the bacteria.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1479
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1753
    Illegal XhoI site found at 1840
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 614
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2587