Difference between revisions of "Part:BBa K4343095"
 
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<partinfo>BBa_K4343095 short</partinfo>
 
<partinfo>BBa_K4343095 short</partinfo>
  
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Short description
 
 
EgElo9 encodes ∆-9 elongase from Euglena gracilis which functions in the Δ-9 elongase/desaturase pathway and could convert C18:2 to C20:2.
 
EgElo9 encodes ∆-9 elongase from Euglena gracilis which functions in the Δ-9 elongase/desaturase pathway and could convert C18:2 to C20:2.
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Description
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====Characterization====
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The EgElo9 gene encodes a protein of 258 amino acids. Heterologous expression of EgElo9 could catalyze linoleic acid (LA, C18:2) to eicosadienoic acid (EDA; C20: 2n-6).
 
The EgElo9 gene encodes a protein of 258 amino acids. Heterologous expression of EgElo9 could catalyze linoleic acid (LA, C18:2) to eicosadienoic acid (EDA; C20: 2n-6).
  
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Reference:
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====Reference====
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Zhu, Q. 365, 307–316 (1999). et al. Metabolic engineering of an oleaginous yeast for the production of omega-3 fatty acids. in Single Cell Oils: Microbial and Algal Oils (eds. Cohen, Z.  
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1. Zhu, Q. 365, 307–316 (1999). et al. Metabolic engineering of an oleaginous yeast for the production of omega-3 fatty acids. in Single Cell Oils: Microbial and Algal Oils (eds. Cohen, Z.  
 
12. & Ratledge, C.) 51–73 (AOCS press, Urbana, 2010).
 
12. & Ratledge, C.) 51–73 (AOCS press, Urbana, 2010).
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===Usage and Biology===
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Latest revision as of 06:19, 9 October 2022


EgElo9

EgElo9 encodes ∆-9 elongase from Euglena gracilis which functions in the Δ-9 elongase/desaturase pathway and could convert C18:2 to C20:2.

Characterization

The EgElo9 gene encodes a protein of 258 amino acids. Heterologous expression of EgElo9 could catalyze linoleic acid (LA, C18:2) to eicosadienoic acid (EDA; C20: 2n-6).

Reference

1. Zhu, Q. 365, 307–316 (1999). et al. Metabolic engineering of an oleaginous yeast for the production of omega-3 fatty acids. in Single Cell Oils: Microbial and Algal Oils (eds. Cohen, Z. 12. & Ratledge, C.) 51–73 (AOCS press, Urbana, 2010).



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 354
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 794