Difference between revisions of "Part:BBa K4343120"

Latest revision as of 05:31, 9 October 2022

EXP

Derived from Y. lipolytica, EXP promotor is a specific DNA sequence upstream of the 5' end of a structural gene that can be specifically recognized and bound by RNAP. This promoter has been used to control the expression of the required enzyme genes in Y. lipolytica to improve the production of omage-3 polyunsaturated fatty acids.

Reference: Xue, Z., Sharpe, P. L., Hong, S. P., Yadav, N. S., Xie, D., Short, D. R., ... & Zhu, Q. (2013). Production of omega-3 eicosapentaenoic acid by metabolic engineering of Yarrowia lipolytica. Nature biotechnology, 31(8), 734-740.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 745
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1
Illegal BsaI.rc site found at 1112

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 __NOTOC__
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 This promoter has been used to control the expression of the required enzyme genes in Y. lipolytica to improve the production of omage-3 polyunsaturated fatty acids.
+Reference:
+Xue, Z., Sharpe, P. L., Hong, S. P., Yadav, N. S., Xie, D., Short, D. R., ... & Zhu, Q. (2013). Production of omega-3 eicosapentaenoic acid by metabolic engineering of Yarrowia lipolytica. Nature biotechnology, 31(8), 734-740.
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