Difference between revisions of "Part:BBa C0051:Experience"
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Our miniprep, double digest and single digest worked as expected. We used this part for further cloning which was successful. The sequencing was correct. However we can not confirm that this part was working as we did not test it's functionality. | Our miniprep, double digest and single digest worked as expected. We used this part for further cloning which was successful. The sequencing was correct. However we can not confirm that this part was working as we did not test it's functionality. | ||
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| + | <partinfo>BBa_C0051 AddReview 1</partinfo> | ||
| + | <I>Michael Lower Warsaw 2009 Team</I> | ||
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| + | Our copy of <partinfo>BBa_C0051</partinfo> was somewhat defective and wasn't able to repress 'cI lam' promoter efficiently enough. | ||
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| + | We have sequenced this brick (results are in 'sequence analysis' tab) and it turned out that it has point mutation C->T at position 450 of cI CDS which leads to formation of premature stop codon at amino acid position 360. Additionally it has two other point mutations S<sub>504</sub>->N and S<sub>786</sub>->C. It leads to truncated nonfunctional protein. We have constructed working version of this brick <partinfo>BBa_K177050</partinfo>. | ||
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| + | |} | ||
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| + | <partinfo>BBa_C0051 AddReview 1</partinfo> | ||
| + | <I>Keegan Owsley UC Davis 2010 team</I> | ||
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| + | Our team encountered some misbehavior with this part; according to our results, a spontaneous promoter is created by the addition of the barcode to the end of this part (steady state expression is roughly 0.32 RPU - 32% level of expression as compared to Bba_J23101). For a version of this part that does not have a barcode (and lacks this transcription), see <partinfo>Bba_K327018</partinfo>. | ||
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Latest revision as of 10:07, 5 August 2017
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_C0051
This part should have a history. Please add content if you have used it.
User Reviews
UNIQf4d71235d5353bee-partinfo-00000000-QINU
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Aberdeen_Scotland 2009 |
Our miniprep, double digest and single digest worked as expected. We used this part for further cloning which was successful. The sequencing was correct. However we can not confirm that this part was working as we did not test it's functionality. |
|
•
Michael Lower Warsaw 2009 Team |
Our copy of BBa_C0051 was somewhat defective and wasn't able to repress 'cI lam' promoter efficiently enough. We have sequenced this brick (results are in 'sequence analysis' tab) and it turned out that it has point mutation C->T at position 450 of cI CDS which leads to formation of premature stop codon at amino acid position 360. Additionally it has two other point mutations S504->N and S786->C. It leads to truncated nonfunctional protein. We have constructed working version of this brick BBa_K177050. |
|
•
Keegan Owsley UC Davis 2010 team |
Our team encountered some misbehavior with this part; according to our results, a spontaneous promoter is created by the addition of the barcode to the end of this part (steady state expression is roughly 0.32 RPU - 32% level of expression as compared to Bba_J23101). For a version of this part that does not have a barcode (and lacks this transcription), see BBa_K327018. |
UNIQf4d71235d5353bee-partinfo-00000007-QINU