Difference between revisions of "Part:BBa K4461010"

 
 
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<partinfo>BBa_K4461010 short</partinfo>
 
<partinfo>BBa_K4461010 short</partinfo>
  
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To insert other proteins that can fusion with mCherry (BBa_J18932), we have to mutate the stop codon for it. Also, for the convenience of inserting proteins, we added cutting site(EcoRI and HindIII) to do enzyme digestion and ligation.
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To insert other proteins that can fusion with mCherry (
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<html>
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<a href="https://parts.igem.org/Part:BBa_J18932">BBa_J18932</a>
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</html>
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), we have to mutate the stop codon for it. Also, for the convenience of inserting proteins, we added cutting site(EcoRI and HindIII) to do enzyme digestion and ligation.
  
  

Latest revision as of 13:16, 11 October 2022


Mut-mCherry + EcoRI and HindIII cutting site

To insert other proteins that can fusion with mCherry ( BBa_J18932 ), we have to mutate the stop codon for it. Also, for the convenience of inserting proteins, we added cutting site(EcoRI and HindIII) to do enzyme digestion and ligation.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 694
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 694
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 694
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 694
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 694
  • 1000
    COMPATIBLE WITH RFC[1000]