Difference between revisions of "Part:BBa K4461010"
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<partinfo>BBa_K4461010 short</partinfo> | <partinfo>BBa_K4461010 short</partinfo> | ||
− | To insert other proteins that can fusion with mCherry (BBa_J18932), we have to mutate the stop codon for it. Also, for the convenience of inserting proteins, we added cutting site(EcoRI and HindIII) to do enzyme digestion and ligation. | + | To insert other proteins that can fusion with mCherry ( |
+ | <html> | ||
+ | <a href="https://parts.igem.org/Part:BBa_J18932">BBa_J18932</a> | ||
+ | </html> | ||
+ | ), we have to mutate the stop codon for it. Also, for the convenience of inserting proteins, we added cutting site(EcoRI and HindIII) to do enzyme digestion and ligation. | ||
Latest revision as of 13:16, 11 October 2022
Mut-mCherry + EcoRI and HindIII cutting site
To insert other proteins that can fusion with mCherry ( BBa_J18932 ), we have to mutate the stop codon for it. Also, for the convenience of inserting proteins, we added cutting site(EcoRI and HindIII) to do enzyme digestion and ligation.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 694
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 694
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 694
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 694
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 694
- 1000COMPATIBLE WITH RFC[1000]