Difference between revisions of "Part:BBa K228866"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K228866 short</partinfo>
 
<partinfo>BBa_K228866 short</partinfo>
  
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T7 polymerase with an amber mutation coding gene is constructed after AraC protein(reversed sequence) and Pbad promoter which would be triggered by Arabinose. T7 polymerase can work well in the exsitence of SupD tRNA. Besides, the expression of the T7 polymerase can be regulated by RBS.
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waiting to be updated
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 07:52, 16 October 2009

AND Gate pBad-SupD pSal-PhiR73 delta(amber mutation)

waiting to be updated


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1274
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2007
    Illegal BamHI site found at 1214
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1839
    Illegal AgeI site found at 1320
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1323