Difference between revisions of "Part:BBa K4244021"

 
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<partinfo>BBa_K4244021 short</partinfo>
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5'UTR of the <i>Echerichia coli</i> cold shock protein <i>cspA</i> gene (1). The PcspA is a regulatory region that has a high rate of transcription but contains a long 5′ UTR of 159 bp that, at 37 °C, acquires an unstable secondary structure and leads to its degradation. On the other hand, at lower temperatures, it forms a stable configuration, which allows translation (2).
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<!-- Add more about the biology of this part here
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4244021 SequenceAndFeatures</partinfo>
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<!-- Uncomment this to enable Functional Parameter display
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===Functional Parameters===
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<partinfo>BBa_K4244021 parameters</partinfo>
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===References===
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1.Mitta, M., Fang, L., & Inouye, M. (1997). Deletion analysis of cspA of Escherichia coli: requirement of the AT-rich UP element for cspA transcription and the downstream box in the coding region for its cold shock induction. Molecular microbiology, 26(2), 321–335. https://doi.org/10.1046/j.1365-2958.1997.5771943.x
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2.Stirling, F., Bitzan, L., O'Keefe, S., Redfield, E., Oliver, J., Way, J., & Silver, P. A. (2017). Rational Design of Evolutionarily Stable Microbial Kill Switches. Molecular cell, 68(4), 686–697.e3. https://doi.org/10.1016/j.molcel.2017.10.033

Latest revision as of 10:17, 12 October 2022


cold shock protein A promoter

5'UTR of the Echerichia coli cold shock protein cspA gene (1). The PcspA is a regulatory region that has a high rate of transcription but contains a long 5′ UTR of 159 bp that, at 37 °C, acquires an unstable secondary structure and leads to its degradation. On the other hand, at lower temperatures, it forms a stable configuration, which allows translation (2).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

1.Mitta, M., Fang, L., & Inouye, M. (1997). Deletion analysis of cspA of Escherichia coli: requirement of the AT-rich UP element for cspA transcription and the downstream box in the coding region for its cold shock induction. Molecular microbiology, 26(2), 321–335. https://doi.org/10.1046/j.1365-2958.1997.5771943.x 2.Stirling, F., Bitzan, L., O'Keefe, S., Redfield, E., Oliver, J., Way, J., & Silver, P. A. (2017). Rational Design of Evolutionarily Stable Microbial Kill Switches. Molecular cell, 68(4), 686–697.e3. https://doi.org/10.1016/j.molcel.2017.10.033