Difference between revisions of "Part:BBa K4180004"
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The enhanced GFP will be used as a control. It will be made into a composite part, pGal1, 10-eGFP-SBP (BBa_K4180008) as a control in the project | The enhanced GFP will be used as a control. It will be made into a composite part, pGal1, 10-eGFP-SBP (BBa_K4180008) as a control in the project | ||
<br> | <br> | ||
| − | (enhanced green fluorescent protein) is used as control | + | eGFP(enhanced green fluorescent protein) is used as a control. The original GFP from the jellyfish Aequorea victoria was not bright enough to show the gene expression or the protein localization of several different organisms. Yang et al lab did several mutations on the GFP DNA sequence to enhance the brightness of GFP to make the detection much more sensitive<br><br> |
| − | The original GFP from the jellyfish Aequorea victoria was not bright enough to show the gene expression or the protein localization of several different organisms. Yang et al lab did several mutations on the GFP DNA sequence to enhance the brightness of GFP to make the detection much more sensitive<br><br> | + | Citation: Yang, T T et al. “Improved fluorescence and dual color detection with enhanced blue and green variants of the green fluorescent protein.” The Journal of biological chemistry vol. 273,14 (1998): 8212-6. doi:10.1074/jbc.273.14.8212<br> |
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
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[[File:BBa K4180004.jpeg|400px|thumb|left|<h3>       BBa K4180004 eGFP | [[File:BBa K4180004.jpeg|400px|thumb|left|<h3>       BBa K4180004 eGFP | ||
<br>         PCR product </h3>]] | <br>         PCR product </h3>]] | ||
| − | [[File:BBa K4180004-2.jpeg|400px|thumb|left|<h3>After Bacteria transformation, bacteria colonies were picking up directly to do eGFP PCR; colony#1 (lane2) has eGFP PCR product </h3>]] | + | [[File:BBa K4180004-2.jpeg|400px|thumb|left|<h3>After Bacteria transformation, bacteria colonies were picking up directly to do eGFP PCR; colony#1 (lane2) has eGFP<br>          PCR product </h3>]] |
Latest revision as of 03:29, 12 October 2022
enhanced GFP (eGFP)
The enhanced GFP will be used as a control. It will be made into a composite part, pGal1, 10-eGFP-SBP (BBa_K4180008) as a control in the project
eGFP(enhanced green fluorescent protein) is used as a control. The original GFP from the jellyfish Aequorea victoria was not bright enough to show the gene expression or the protein localization of several different organisms. Yang et al lab did several mutations on the GFP DNA sequence to enhance the brightness of GFP to make the detection much more sensitive
Citation: Yang, T T et al. “Improved fluorescence and dual color detection with enhanced blue and green variants of the green fluorescent protein.” The Journal of biological chemistry vol. 273,14 (1998): 8212-6. doi:10.1074/jbc.273.14.8212
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]