Difference between revisions of "Part:BBa K4268001:Experience"
 
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[[File: T-suny-oneonta-t7-capsid assembly protein colony PCR figure.png|500px|thumb|center|Figure 1: Colony PCR of five colonies (clones) suspected of coating the insert Capsid Assembly Protein insert. The insert length is 768bp and the predicted size of the PCR product when using VF/VR primers is 934 bp.]]
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[[File: T-suny-oneonta-t7-capsid assembly protein colony PCR figure.png|500px|thumb|center|Figure 1: Colony PCR of five colonies (clones) suspected of containing the Capsid Assembly Protein insert. The insert length is 768bp and the predicted size of the PCR product when using VF/VR primers is 1073 bp.]]
  
 
The gel indicates that colonies 1 and 2 are likely to contain the correct insert and thus, the Capsid Assembly Protein was successfully cloned into a Level 0 Golden Gate Assembly basic part.
 
The gel indicates that colonies 1 and 2 are likely to contain the correct insert and thus, the Capsid Assembly Protein was successfully cloned into a Level 0 Golden Gate Assembly basic part.
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Latest revision as of 18:21, 8 October 2022


The part was received, resuspended, and cloned into the level 0 Golden Gate vector, PSB1C00. The ligation was then transformed into DH5α cells. After overnight growth, white colonies (indicative of a replacement of the RFP reporter cassette) were subjected to screening for the correct insert using colony PCR with the VF/VR primer pair.


Figure 1: Colony PCR of five colonies (clones) suspected of containing the Capsid Assembly Protein insert. The insert length is 768bp and the predicted size of the PCR product when using VF/VR primers is 1073 bp.

The gel indicates that colonies 1 and 2 are likely to contain the correct insert and thus, the Capsid Assembly Protein was successfully cloned into a Level 0 Golden Gate Assembly basic part.


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