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===Design Notes===
 
===Design Notes===
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By using a RNA-based gene-regulatory platform, we design a ribozyme switch linked behind YopE mRNA, it contains a sensor domain, comprised of an aptamer sequence, and an actuator domain, comprised of hammerhead ribozyme sequence.  
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By using a RNA-based gene-regulatory platform, we design a riboswitch contained a sensor domain, comprised of an aptamer sequence, and an actuator domain, comprised of hammerhead ribozyme sequence.  
 
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<img src="https://2020.igem.org/wiki/images/7/7e/T--OUC-China--result_fig2_th.jpg" style="width: 80%;">
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===Source===
 
===Source===

Latest revision as of 11:30, 8 October 2022


ribozyme riboswitch


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 38
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 38
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 38
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 38
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

By using a RNA-based gene-regulatory platform, we design a riboswitch contained a sensor domain, comprised of an aptamer sequence, and an actuator domain, comprised of hammerhead ribozyme sequence.

Source

synthesize from company

References

Win, M.N. and Smolke, C.D. (2007). A modular and extensible RNA-based gene-regulatory platform for engineering cellular function. Proceedings of the National Academy of Sciences, 104(36), pp.14283–14288. doi:10.1073/pnas.0703961104.