Difference between revisions of "Part:BBa K4193002:Design"
(→‎Design Notes)
 
(3 intermediate revisions by the same user not shown)
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
By using a RNA-based gene-regulatory platform, we design a ribozyme switch linked behind YopE mRNA, it contains a sensor domain, comprised of an aptamer sequence, and an actuator domain, comprised of hammerhead ribozyme sequence.  
+
By using a RNA-based gene-regulatory platform, we design a riboswitch contained a sensor domain, comprised of an aptamer sequence, and an actuator domain, comprised of hammerhead ribozyme sequence.  
 
<html>
 
<html>
 
<head>
 
<head>
Line 15: Line 15:
 
<body>
 
<body>
 
<div>
 
<div>
−
<img src="https://2020.igem.org/wiki/images/7/7e/T--OUC-China--result_fig2_th.jpg" style="width: 80%;">
+
<img src="">
 
</div>
 
</div>
 
</body>
 
</body>
 
</html>
 
</html>
−
 
−
 
  
 
===Source===
 
===Source===
  
−
not yet
+
synthesize from company
  
 
===References===
 
===References===
 +
Win, M.N. and Smolke, C.D. (2007). A modular and extensible RNA-based gene-regulatory platform for engineering cellular function. Proceedings of the National Academy of Sciences, 104(36), pp.14283–14288. doi:10.1073/pnas.0703961104.

Latest revision as of 11:30, 8 October 2022


ribozyme riboswitch


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 38
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 38
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 38
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 38
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

By using a RNA-based gene-regulatory platform, we design a riboswitch contained a sensor domain, comprised of an aptamer sequence, and an actuator domain, comprised of hammerhead ribozyme sequence.

Source

synthesize from company

References

Win, M.N. and Smolke, C.D. (2007). A modular and extensible RNA-based gene-regulatory platform for engineering cellular function. Proceedings of the National Academy of Sciences, 104(36), pp.14283–14288. doi:10.1073/pnas.0703961104.