Difference between revisions of "Part:BBa K4137007:Design"

Latest revision as of 07:51, 10 October 2022

pLac+RBS+CcdB-Myc+B1006

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 271

Design Notes

RBS is used to enhance ccdB synthesis. pLac promoter is chosen for constitutive expression of ccdB. The fusion of ccdB and myc epitope tag allows the isolation of ccdB from the ccdB-ccdA complex upon purification for easy identification of individual concentrations.

Source

https://www.ncbi.nlm.nih.gov/nuccore/U51588.1 https://sci-hub.hkvisa.net/10.1016/0022-2836(85)90070-1 https://2018.igem.org/Team:Pasteur_Paris/Kill

@@ Line 10: / Line 10: @@
 pLac promoter is chosen for constitutive expression of ccdB.
 The fusion of ccdB and myc epitope tag allows the isolation of ccdB from the ccdB-ccdA complex upon purification for easy identification of individual concentrations.
@@ Line 16: / Line 17: @@
 ===Source===
-https://www.ncbi.nlm.nih.gov/nuccore/X75982.1
-https://www.snapgene.com/resources/plasmid-files/?set=pet_and_duet_vectors_(novagen)&plasmid=pET-28a(%2B)
 https://www.ncbi.nlm.nih.gov/nuccore/U51588.1
-https://www.snapgene.com/resources/plasmid-files/?set=pet_and_duet_vectors_(novagen)&plasmid=pET-28a(%2B)
+https://sci-hub.hkvisa.net/10.1016/0022-2836(85)90070-1
-https://www.snapgene.com/resources/plasmid-files/?set=pet_and_duet_vectors_(novagen)&plasmid=pET-28a(%2B)
+https://2018.igem.org/Team:Pasteur_Paris/Kill
-https://journals.asm.org/doi/10.1128/jb.171.6.3108-3114.1989
 ===References===