Difference between revisions of "Part:BBa K4137013:Design"
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The fusion of ccdB and myc epitope tag allows the isolation of ccdB from the ccdB-ccdA complex upon purification for easy identification of individual concentrations. The 6xHis tag is used for protein quantification and is the most commonly used purification his-tag. | The fusion of ccdB and myc epitope tag allows the isolation of ccdB from the ccdB-ccdA complex upon purification for easy identification of individual concentrations. The 6xHis tag is used for protein quantification and is the most commonly used purification his-tag. | ||
− | + | [[File:ccdb-tag-linear.png|800px|thumb|center|Fig.1 Benching linear map of final ccdB construct.]] | |
Latest revision as of 07:52, 10 October 2022
ccdB toxin secretion pathway
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 289
Design Notes
pLac promoter is chosen for constitutive expression of ccdB. The fusion of ccdB and myc epitope tag allows the isolation of ccdB from the ccdB-ccdA complex upon purification for easy identification of individual concentrations. The 6xHis tag is used for protein quantification and is the most commonly used purification his-tag.
Source
https://www.ncbi.nlm.nih.gov/nuccore/U51588.1 https://sci-hub.hkvisa.net/10.1016/0022-2836(85)90070-1 https://2018.igem.org/Team:Pasteur_Paris/Kill