Difference between revisions of "Part:BBa K4137006:Design"
(4 intermediate revisions by 2 users not shown) | |||
Line 1: | Line 1: | ||
+ | __NOTOC__ | ||
+ | <partinfo>BBa_K4137006 short</partinfo> | ||
+ | |||
+ | |||
+ | <partinfo>BBa_K4137006 SequenceAndFeatures</partinfo> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | ===Design Notes=== | ||
We chose to use p_mles, the mleR regulatory site as mleR can be induced by malate acid which surrounds the tree roots. CcdA is the antitoxin of the toxin Ccdb which kills chi18h8 when the probiotic isn’t near the roots of the banana tree. The 6xHis is used for protein quantification and is the most commonly used purification his-tag. | We chose to use p_mles, the mleR regulatory site as mleR can be induced by malate acid which surrounds the tree roots. CcdA is the antitoxin of the toxin Ccdb which kills chi18h8 when the probiotic isn’t near the roots of the banana tree. The 6xHis is used for protein quantification and is the most commonly used purification his-tag. | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | ===Source=== | ||
+ | |||
+ | |||
+ | https://www.snapgene.com/resources/plasmid-files/?set=pet_and_duet_vectors_(novagen)&plasmid=pET-28a(%2B) | ||
+ | https://www.snapgene.com/resources/plasmid-files/?set=pet_and_duet_vectors_(novagen)&plasmid=pET-28a(%2B) | ||
+ | https://parts.igem.org/Part:BBa_K1659003 | ||
+ | |||
+ | |||
+ | |||
+ | ===References=== |
Latest revision as of 08:24, 10 October 2022
CcdA expressing construct
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We chose to use p_mles, the mleR regulatory site as mleR can be induced by malate acid which surrounds the tree roots. CcdA is the antitoxin of the toxin Ccdb which kills chi18h8 when the probiotic isn’t near the roots of the banana tree. The 6xHis is used for protein quantification and is the most commonly used purification his-tag.
Source
https://www.snapgene.com/resources/plasmid-files/?set=pet_and_duet_vectors_(novagen)&plasmid=pET-28a(%2B) https://www.snapgene.com/resources/plasmid-files/?set=pet_and_duet_vectors_(novagen)&plasmid=pET-28a(%2B) https://parts.igem.org/Part:BBa_K1659003