Difference between revisions of "Part:BBa K228256"

Latest revision as of 14:15, 17 October 2009

AND GATE AraC+SupD+Sal+RBS(B0031)+T7ptag

This Device belongs to the SupD_T7ptag AND Gate family constructed by Peking University 2009 iGEM Team

Use arabinose to induce the transcription of SupD and use salicylate to induce the transcription of T7ptag. If the SupD tRNA is existent, T7ptag can be translated into functional T7 polymerase, and otherwise the translation of T7ptag will come to stop because of some amber stop codon mutations in the T7ptag. The T7 polymerase will activate a T7 promoter.

This gate is only one of nine gates(K228255-K228263), which are different in the RBS of T7ptag.

For more information: refer to K228000(T7ptag) and K228001(SupD) referrence: Anderson JC, Voigt CA, Arkin AP (2007) Environmental signal integration by a modular AND gate. Mol Syst Biol 3: 133

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1274
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 2144
Illegal BamHI site found at 1214
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1976
Illegal AgeI site found at 1320
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1323

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K228256 short</partinfo>
+{{PKU_Beijing2009_AND_GATE_FAMILY}}
+Use arabinose to induce the transcription of SupD and use salicylate to induce the transcription of T7ptag. If the SupD tRNA is existent, T7ptag can be translated into functional T7 polymerase, and otherwise the translation of T7ptag will come to stop because of some amber stop codon mutations in the T7ptag. The T7 polymerase will activate a T7 promoter.
+This gate is only one of nine gates(K228255-K228263), which are different in the RBS of T7ptag.
+For more information:
+refer to K228000(T7ptag) and K228001(SupD)
+referrence: Anderson JC, Voigt CA, Arkin AP (2007) Environmental signal integration by a modular AND gate. Mol Syst Biol 3: 133
 <!-- Add more about the biology of this part here