Difference between revisions of "Part:BBa K4368004"

Latest revision as of 18:54, 7 October 2022

pcI + rbs + glgC16 + terminator

Description

GlgC16 encodes for the ADP-glucose pyrophosphorylase gene of Escherichia coli (BBa_K118016). This enzyme is responsible for the synthesis of starch-like starch using glucose as a substrate. In addition, this part includes the composition used by the team, which includes a strong rbs (BBa_B0030), a double terminator (BBa_B0015) as well as a promoter inducible by the cI protein of the phage lambda (BBa_R1051). BBa_K118016 works under the control of this promoter to build the glucose concentration-based gene regulatory circuit that integrates all our parts.

Characterization

The expression cassette sequence was digested with EcoRI and PstI enzymes and subsequently ligated with a chloramphenicol resistant plasmid backbone (Cm). Transforming bacteria were created with this plasmid and seeded on LB-Agar+Cm plates. After growth, colonies were selected based on their color (white) and DNA extraction was performed using the Promega PureYield Plasmid Miniprep System kit. The resulting DNA is used for further digestion with EcoRI and PstI. The digests are then run on a 0.75% agarose gel at 90 mV voltage and constant amperage. BioRad brand RedSafe is used as an intercalating developing agent.

Enzyme digestion

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 272
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Contribution

Group: UMA_MALAGA
Author: Jiménez Amores, Carmen

@@ Line 4: / Line 4: @@
 ==Description==
-''GlgC16'' encodes for the ADP-glucose pyrophosphorylase gene of ''Escherichia coli'' (<partinfo>BBa_K118016</partinfo>). This enzyme is responsible for the synthesis of starch-like starch using glucose as a substrate. In addition, this part includes the composition used by the team, which includes a strong rbs (<partinfo>BBa_B0030</partinfo>), a double terminator (<partinfo>BBa_B0015</partinfo>) as well as a promoter inducible by the cI protein of the phage lambda (<partinfo>BBa_R1051</partinfo>).
+''GlgC16'' encodes for the ADP-glucose pyrophosphorylase gene of ''Escherichia coli'' (<partinfo>BBa_K118016</partinfo>). This enzyme is responsible for the synthesis of starch-like starch using glucose as a substrate. In addition, this part includes the composition used by the team, which includes a strong rbs (<partinfo>BBa_B0030</partinfo>), a double terminator (<partinfo>BBa_B0015</partinfo>) as well as a promoter inducible by the ''cI'' protein of the phage lambda (<partinfo>BBa_R1051</partinfo>).
 <partinfo>BBa_K118016</partinfo> works under the control of this promoter to build the glucose concentration-based gene regulatory circuit that integrates all our parts.