Difference between revisions of "Part:BBa K4345016"
 
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<partinfo>BBa_K4345016 short</partinfo>
 
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CcdB protein is toxin of type II toxin-antitoxin system CcdB/CcdA. CcdB is a toxin that kills E. coli by targeting the GyrA subunit of DNA gyrase, an essential type II topoisomerase (Bahassi et al., 1999). In this part, we added three stop codons to prevent the translation and be able to clone CcdB into our host.
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CcdB protein is toxin of type II toxin-antitoxin system CcdB/CcdA. CcdB is a toxin that kills ''E. coli'' by targeting the GyrA subunit of DNA gyrase, an essential type II topoisomerase (Bahassi et al., 1999). In this part, we added three stop codons, one in each reading frame, to prevent the translation and be able to clone CcdB into our host. The stop codons are flanked by SalI restriction sites.
  
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===Usage and Biology===
 
  
 
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<span class='h3bb'>Sequence and Features</span>
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===Sequence and Features===
 
<partinfo>BBa_K4345016 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4345016 SequenceAndFeatures</partinfo>
  
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===References===
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Bahassi, E. M., O’Dea, M. H., Allali, N., Messens, J., Gellert, M., & Couturier, M. (1999, April). Interactions of CcdB with DNA Gyrase. Journal of Biological Chemistry, 274(16), 10936–10944. https://doi.org/10.1074/jbc.274.16.10936
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===Functional Parameters===
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<partinfo>BBa_K4345016 parameters</partinfo>
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Latest revision as of 13:51, 8 October 2022


ccdB with STOP codon control

CcdB protein is toxin of type II toxin-antitoxin system CcdB/CcdA. CcdB is a toxin that kills E. coli by targeting the GyrA subunit of DNA gyrase, an essential type II topoisomerase (Bahassi et al., 1999). In this part, we added three stop codons, one in each reading frame, to prevent the translation and be able to clone CcdB into our host. The stop codons are flanked by SalI restriction sites.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 249
  • 1000
    COMPATIBLE WITH RFC[1000]

References

Bahassi, E. M., O’Dea, M. H., Allali, N., Messens, J., Gellert, M., & Couturier, M. (1999, April). Interactions of CcdB with DNA Gyrase. Journal of Biological Chemistry, 274(16), 10936–10944. https://doi.org/10.1074/jbc.274.16.10936