Difference between revisions of "Part:BBa K4140027"

(→‎Usage)
(→‎Usage)
 
(3 intermediate revisions by 2 users not shown)
Line 4: Line 4:
  
 
==Part Description==
 
==Part Description==
−
In this composite Lac promoter regulate the expression of phenylalanine permease that is a single, integral membrane transporter that uses the proton motive force to antiport L-phenylalanine and L-tyrosine. And present naturally in Escherichia coli.
+
In this composite, the Lac promoter regulates the expression of phenylalanine permease which is a single integral membrane transporter that uses the proton motive force to antiport L-phenylalanine and L-tyrosine. And present naturally in Escherichia coli.
  
 
==Usage==
 
==Usage==
  
−
We implement this composite to increase the permeability of cells to phenylalanine by the expression of phenylalanine permease that is a single, integral membrane transporter to improve the sensitivity of our circuit to phenylalanine as shown in figure 1 and figure 2.
+
We implement this composite to increase the permeability of cells to phenylalanine by the expression of phenylalanine permease which is a single, integral membrane transporter to improve the sensitivity of our circuit to phenylalanine as shown in figure 1 and figure 2.
  
 
[[File:per.png|Right ]]
 
[[File:per.png|Right ]]
  
−
Figure 1. (shows the permeability circuit in our whole cell-based biosensor.)
+
Figure 1. (shows the permeability circuit in our E.coli-based system.)
  
 
[[File:wcb.png|Right ]]
 
[[File:wcb.png|Right ]]
Line 18: Line 18:
 
Figure 2. (shows the whole cell-based biosensor.)
 
Figure 2. (shows the whole cell-based biosensor.)
  
−
+
==Experimental Characterization==
 +
[[File:tube131.png|right|]]
 +
<br><br><br><br><br><br><br>
 +
This figure shows an experimental characterization of this part as it's validated through gel electrophoresis as it is in lane 7. The run part (ordered from IDT) included Lac promoter - Permease.
 +
<br><br><br><br><br><br><br><br><br><br><br><br><br><br>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 04:51, 12 October 2022


Permeability device

Part Description

In this composite, the Lac promoter regulates the expression of phenylalanine permease which is a single integral membrane transporter that uses the proton motive force to antiport L-phenylalanine and L-tyrosine. And present naturally in Escherichia coli.

Usage

We implement this composite to increase the permeability of cells to phenylalanine by the expression of phenylalanine permease which is a single, integral membrane transporter to improve the sensitivity of our circuit to phenylalanine as shown in figure 1 and figure 2.

Right

Figure 1. (shows the permeability circuit in our E.coli-based system.)

Right

Figure 2. (shows the whole cell-based biosensor.)

Experimental Characterization

Tube131.png








This figure shows an experimental characterization of this part as it's validated through gel electrophoresis as it is in lane 7. The run part (ordered from IDT) included Lac promoter - Permease.













Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 81
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]