Difference between revisions of "Part:BBa K4268002:Experience"
 
(4 intermediate revisions by the same user not shown)
Line 4: Line 4:
  
  
−
[[File: T-suny-oneonta-t7-capsid protein colony PCR figure.png|500px|thumb|center|Figure 1: Colony PCR of five colonies (clones) suspected of coating the insert capsid assembly protein insert. The insert length is 769bp and the predicted size of the PCR product when using VF/VR primers is 1739 bp.]]
+
[[File: T-suny-oneonta-t7-capsid protein colony PCR figure.png|500px|thumb|center|Figure 1: Colony PCR of five colonies (clones) suspected of containing the Capsid Protein insert. The insert length is 720bp and the predicted size of the PCR product when using VF/VR primers is 1025 bp.]]
  
−
The gel indicates that all five colonies are likely to contain the correct insert and thus, the _ was successfully cloned into a Level O Golden Gate Assembly basic part.
+
The gel indicates that all five colonies are likely to contain the correct insert and thus, the Capsid Protein was successfully cloned into a Level 0 Golden Gate Assembly basic part.
  
 
===Applications of BBa_K4268002===
 
===Applications of BBa_K4268002===
Line 19: Line 19:
 
<I>Username</I>
 
<I>Username</I>
 
|width='60%' valign='top'|
 
|width='60%' valign='top'|
−
Enter the review inofrmation here.
+
Enter the review information here.
 
|};
 
|};
 
<!-- End of the user review template -->
 
<!-- End of the user review template -->
 
<!-- DON'T DELETE --><partinfo>BBa_K4268002 EndReviews</partinfo>
 
<!-- DON'T DELETE --><partinfo>BBa_K4268002 EndReviews</partinfo>

Latest revision as of 18:20, 8 October 2022


The part was received, resuspended, and cloned into the level 0 Golden Gate vector, PSB1C00. The ligation was then transformed into DH5α cells. After overnight growth, white colonies (indicative of a replacement of the RFP reporter cassette) were subjected to screening for the correct insert using colony PCR with the VF/VR primer pair.


Figure 1: Colony PCR of five colonies (clones) suspected of containing the Capsid Protein insert. The insert length is 720bp and the predicted size of the PCR product when using VF/VR primers is 1025 bp.

The gel indicates that all five colonies are likely to contain the correct insert and thus, the Capsid Protein was successfully cloned into a Level 0 Golden Gate Assembly basic part.

Applications of BBa_K4268002

User Reviews

UNIQbbb18dfd7f317be2-partinfo-00000000-QINU UNIQbbb18dfd7f317be2-partinfo-00000001-QINU