Difference between revisions of "Part:BBa K4156112"

 
 
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<partinfo>BBa_K4156112 short</partinfo>
 
<partinfo>BBa_K4156112 short</partinfo>
  
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plac-LuxCDABE is a complex regulatory system of plac operon (<html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/Part:BBa_R0010"> BBa_R0010 </a></html> ) and LuxCDABE operon We designed plac-LuxCDABE as a control for observing the abundance level of bacteria.
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===Usage and Biology===
 
===Usage and Biology===
  
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In this biobrick, we fused the plac(<html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/Part: BBa_K4156079"> BBa_K4156079 </a></html> )  with the LuxCDABE (<html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/Part: BBa_K4156095"> BBa_K4156095 </a></html> )  and introduced the RiboJ sequence and RBS B0034 in between. this biobrick was used to facilitate the observation of bacterial abundance changes in tumor cells while avoiding the environmental interference associated with the use of mRFP.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K4156112 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4156112 SequenceAndFeatures</partinfo>

Latest revision as of 13:54, 11 October 2022


plac-LuxCDABE

plac-LuxCDABE is a complex regulatory system of plac operon ( BBa_R0010 ) and LuxCDABE operon We designed plac-LuxCDABE as a control for observing the abundance level of bacteria.


Usage and Biology

In this biobrick, we fused the plac( BBa_K4156079 ) with the LuxCDABE ( BBa_K4156095 ) and introduced the RiboJ sequence and RBS B0034 in between. this biobrick was used to facilitate the observation of bacterial abundance changes in tumor cells while avoiding the environmental interference associated with the use of mRFP.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 328
    Illegal BglII site found at 2309
    Illegal BglII site found at 5561
    Illegal BamHI site found at 872
    Illegal BamHI site found at 5861
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 741
    Illegal NgoMIV site found at 1368
    Illegal AgeI site found at 5879
  • 1000
    COMPATIBLE WITH RFC[1000]