Difference between revisions of "Part:BBa K4156098"

 
 
(5 intermediate revisions by 2 users not shown)
Line 3: Line 3:
 
<partinfo>BBa_K4156098 short</partinfo>
 
<partinfo>BBa_K4156098 short</partinfo>
  
−
To be refined
+
pCadC-Bxb1-φ174E is a complex component expressing the cleavage gene φ174E, constructed from the pH-sensitive promoter pCadC and the serine integrase Bxb1.
 +
 
 +
<!-- Add more about the biology of this part here -->
  
−
<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
  
−
<!-- -->
+
We designed pCadC-Bxb1-φ174E to test the expression efficiency of φ174E under the control of a logic gate coupling the pCadC promoter to Bxb1. We will validate the function of this biobrick by measuring bacterial viability.
 +
 
 +
 
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K4156098 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4156098 SequenceAndFeatures</partinfo>

Latest revision as of 12:08, 11 October 2022


pCadC-Bxb1-φ174E

pCadC-Bxb1-φ174E is a complex component expressing the cleavage gene φ174E, constructed from the pH-sensitive promoter pCadC and the serine integrase Bxb1.


Usage and Biology

We designed pCadC-Bxb1-φ174E to test the expression efficiency of φ174E under the control of a logic gate coupling the pCadC promoter to Bxb1. We will validate the function of this biobrick by measuring bacterial viability.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 368
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 642
    Illegal XhoI site found at 729
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1476