Difference between revisions of "Part:BBa K4225002:Design"

 
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===Design Notes===
 
===Design Notes===
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Prefix and Suffix are added, and spacers are also added between the parts.
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Prefix-Suffix are added to our sequence and spacers are also added between the parts. This composite part is also codon optimized for our chassis, DH5α.
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+
−
 
+
  
 
===Source===
 
===Source===
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−
ATCGAGAATTCGCGGCCGCTTCTAGAGTTCATTATCCATCCTCCATCGCCACGATAGTTCATGGCGATAGGTAGAATAGCAATGAACGATTATCCCTATCAAGCATTCTGACTGAGCATTGCTCACAACTTTATCTGAGAATAGTCAATCTTCGGAAATCCCAGGTGAAAGAGGAGAAATAATAAAAGGTCCCGATGCGCAAGGGTGAGGAGCTTTTCACCGGCGTCGTGCCTATCTTGGTCGAGCTGGACGGCGACGTTAATGGGCACAAGTTCTCAGTCAGCGGGGAAGGGGAAGGTGACGCGACATACGGGAAACTGACCCTTAAATTTATTTGTACGACAGGCAAATTACCAGTTCCTTGGCCCACACTGGTGACTACGTTTGGTTATGGTGTGCAGTGTTTTGCTCGCTATCCGGATCATATGAAGCAGCATGATTTTTTCAAGTCAGCTATGCCCGAAGGTTATGTACAAGAGCGTACTATCTTTTTCAAGGATGATGGAAATTATAAAACGCGTGCTGAGGTTAAATTTGAGGGGGATACACTGGTAAACCGCATTGAATTAAAAGGCATTGATTTCAAGGAAGACGGGAATATTCTGGGTCACAAGTTAGAGTACAACTATAATTCGCATAACGTCTATATCATGGCAGACAAACAGAAGAACGGAATTAAGGTAAACTTTAAGATCCGCCACAATATCGAGGACGGTAGTGTCCAACTTGCGGACCACTACCAGCAAAACACACCAATTGGCGATGGGCCTGTTTTATTGCCAGACAACCACTACCTTAGCACACAATCTGCGCTGTCGAAAGATCCAAACGAGAAGCGTGATCACATGGTCCTGCTTGAGTTCGTGACAGCAGCAGGTATCACCCACGGTATGGACGAGTTGTACAAGTGATAAAGATTACTACTGATACCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCTACTAGAGTCACACTGGCTCACCTTCGGGTGGGCCTTTCTGCGTTTATATACTAGTAGCGGCCGCTGCAGATCGA
 
  
 
===References===
 
===References===

Latest revision as of 14:32, 12 October 2022


GFP that is induced by oxySp, a H2O2 inducible promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 811


Design Notes

Prefix-Suffix are added to our sequence and spacers are also added between the parts. This composite part is also codon optimized for our chassis, DH5α.

Source

References