Difference between revisions of "Part:BBa K4162001"
 
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===Introduction===
 
===Introduction===
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[[File:T--Fudan--logo-Rester-rectangle.jpg|100px|right|2021 Fudan]]
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[[File:T--Fudan--logo-Rester-rectangle.jpg|100px|right|2022 Fudan]]
  
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A new version of er-(n2)oxStayGold(c4), which gives a higher expression level. The StayGold gene has codon optimization for mammalian expression. For brighter ER imaging if permitted
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A new version of StayGold<ref>A highly photostable and bright green fluorescent protein.
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Hirano M,  Ando R,  Shimozono S,  Sugiyama M,  Takeda N,  Kurokawa H,  Deguchi R,  Endo K,  Haga K,  Takai-Todaka R,  Inaura S,  Matsumura Y,  Hama H,  Okada Y,  Fujiwara T,  Morimoto T,  Katayama K,  Miyawaki A.
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Nat Biotechnol, 2022 Jul;40(7):1132-1142.  PMID:35468954</ref>, which gives a higher expression level than the original version, and  is over one order of magnitude more photostable than EGFP. The StayGold gene has been codon optimization for exogenous expression.
  
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===Worth to Notice===
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This biobrick is the version of StayGold available on [https://www.addgene.org/186296/ Addgene].
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===Characterization===
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[[File:T--Fudan--micro--m2-BCMO-ybbO.png|400px|thumb|none|'''Figure 1. '''Fluorescent imaging confirm the expression of both BCMO and ybbO. We observed fluorescence images of ''E. coli'' (1. Bacteria expressing BCMO-linker-StayGold ; 2. Bacteria expressing BCMO and ybbO-tdMCP-GFP ) through an Olympus fluorescence microscope with a 1.45 NA 150× oil objective. GFP and StayGold were excited by a 488 nm laser. Images were taken by the same settings for comparison.]]
  
 
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Latest revision as of 16:13, 13 October 2022


StayGold = (n2)oxStayGold(c4)v2.0


Introduction

2022 Fudan

A new version of StayGold[1], which gives a higher expression level than the original version, and is over one order of magnitude more photostable than EGFP. The StayGold gene has been codon optimization for exogenous expression.

Worth to Notice

This biobrick is the version of StayGold available on Addgene.

Characterization

Figure 1. Fluorescent imaging confirm the expression of both BCMO and ybbO. We observed fluorescence images of E. coli (1. Bacteria expressing BCMO-linker-StayGold ; 2. Bacteria expressing BCMO and ybbO-tdMCP-GFP ) through an Olympus fluorescence microscope with a 1.45 NA 150× oil objective. GFP and StayGold were excited by a 488 nm laser. Images were taken by the same settings for comparison.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 638
    Illegal BsaI.rc site found at 658


  1. ↑ A highly photostable and bright green fluorescent protein. Hirano M,  Ando R,  Shimozono S,  Sugiyama M,  Takeda N,  Kurokawa H,  Deguchi R,  Endo K,  Haga K,  Takai-Todaka R,  Inaura S,  Matsumura Y,  Hama H,  Okada Y,  Fujiwara T,  Morimoto T,  Katayama K,  Miyawaki A. Nat Biotechnol, 2022 Jul;40(7):1132-1142. PMID:35468954