Difference between revisions of "Part:BBa K4298012:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | pUC19 backbone, using Gibson assembly insert the EPI with α-secretion signal. | |
===Source=== | ===Source=== | ||
| − | + | Assembly with K4298009, K319003, K3105674, K4298005, K731721 and K4298011. | |
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Latest revision as of 08:43, 7 October 2022
pUC19-ITR-EPI
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1182
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1000
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 505
Design Notes
pUC19 backbone, using Gibson assembly insert the EPI with α-secretion signal.
Source
Assembly with K4298009, K319003, K3105674, K4298005, K731721 and K4298011.