Difference between revisions of "Part:BBa K4197015"
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<h2>Introduction</h2> | <h2>Introduction</h2> | ||
| − | <p>This part is composed of the gene coding for the allergen of dust mite Der p 1 (NCBI: <a "https://www.ncbi.nlm.nih.gov/nuccore/U11695.1/">U11695.1</a>). The mite allergy prevalence is 20% ( | + | <p>This part is composed of the gene coding for the allergen of dust mite Der p 1 (NCBI: <a "https://www.ncbi.nlm.nih.gov/nuccore/U11695.1/">U11695.1</a>). The mite allergy prevalence is 20% (Lieberman and al. 2018) in the US and Der p 1 triggers 87% of dust mite allergies (Mueller and al. 2015). Der p 1 have already been expressed in <i>E. coli </i>and was able to bind the IgE of patient with peanut's allergie (Bussières and al. 2010). </p> |
<h2>Construction</h2> | <h2>Construction</h2> | ||
| − | <p>The objective of the INSA-UPS 2022 team was to display Der p 1 on the surface of <i>E. coli</i> so the ordered sequence was merged to OmpA membrane | + | <p>The objective of the INSA-UPS 2022 team was to display Der p 1 on the surface of <i>E. coli</i> so the ordered sequence was merged to OmpA membrane lipoprotein (see part <a href="https://parts.igem.org/Part:BBa_K4197006">BBa_K1694006</a>).</p> |
| Line 22: | Line 22: | ||
- <a href="https://parts.igem.org/Part:BBa_K4197008"> OmpA_Ara h 2</a> <br> | - <a href="https://parts.igem.org/Part:BBa_K4197008"> OmpA_Ara h 2</a> <br> | ||
- <a href="https://parts.igem.org/Part:BBa_K4197007"> OmpA_Ana o 3</a> <br> | - <a href="https://parts.igem.org/Part:BBa_K4197007"> OmpA_Ana o 3</a> <br> | ||
| − | - <a href="https://parts.igem.org/Part: | + | - <a href="https://parts.igem.org/Part:BBa_K4197009"> OmpA_Gal d 2</a> <br> |
</p> | </p> | ||
Latest revision as of 08:24, 8 October 2022
Gene coding for Der p 1
Gene coding for Mite allergen called Der p 1.
Introduction
This part is composed of the gene coding for the allergen of dust mite Der p 1 (NCBI: U11695.1). The mite allergy prevalence is 20% (Lieberman and al. 2018) in the US and Der p 1 triggers 87% of dust mite allergies (Mueller and al. 2015). Der p 1 have already been expressed in E. coli and was able to bind the IgE of patient with peanut's allergie (Bussières and al. 2010).
Construction
The objective of the INSA-UPS 2022 team was to display Der p 1 on the surface of E. coli so the ordered sequence was merged to OmpA membrane lipoprotein (see part BBa_K1694006).
References
More information about the project for which the part was created: DAISY (INSA-UPS 2022)
Other parts to display allergens:
- OmpA_Ara h 2
- OmpA_Ana o 3
- OmpA_Gal d 2
- Mueller, G. A.; Randall, T. A.; Glesner, J.; Pedersen, L. C.; Perera, L.; Edwards, L. L.; DeRose, E. F.; Chapman, M. D.; London, R. E.; Pomés, A. (2016). Serological, genomic and structural analyses of the major mite allergen Der p 23. Clinical & Experimental Allergy, 46(2), 365–376. doi:10.1111/cea.12680
- Bussières, L., Bordas-Le Floch, V., Bulder, I., Chabre, H., Nony, E., Lautrette, A., Berrouet, C., Nguefeu, Y., Horiot, S., Baron-Bodo, V., Van Overtvelt, L., De Conti, A. M., Schlegel, A., Maguet, N., Mouz, N., Lemoine, P., Batard, T., & Moingeon, P. (2010). Recombinant Fusion Proteins Assembling Der p 1 and Der p 2 Allergens from Dermatophagoides pteronyssinus. International Archives of Allergy and Immunology, 153(2), 141–151. https://doi.org/10.1159/000312631
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]