Difference between revisions of "Part:BBa K4245003:Design"

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===Design Notes===
 
===Design Notes===
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The iSpinach aptamer was originally generated by the researchers at the University of Strasbourg using the SELEX (systematic evolution of ligands by exponential enrichment) process (Millan et. al, 2017).
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This part works identically to <partinfo>BBa_K4245001</partinfo>, a composite part producing Spinach, and <partinfo>BBa_K4245002</partinfo>, a composite part producing iSpinach; the only difference is the aptamer produced. This disparity allows accurate comparison of the basic aptamer parts.
  
 
===Source===
 
===Source===
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===References===
 
===References===
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Autour, A., Westhof, E., & Ryckelynck, M. (2016). iSpinach: A fluorogenic RNA aptamer optimized for in vitro applications. Nucleic Acids Research, 44(6), 2491–2500. https://doi.org/10.1093/nar/gkw083
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Fernandez-Millan, P., Autour, A., Ennifar, E., Westhof, E., & Ryckelynck, M. (2017). Crystal structure and fluorescence properties of the iSpinach aptamer in complex with DFHBI. RNA, 23(12), 1788–1795. https://doi.org/10.1261/rna.063008.117
  
 
Neubacher, S., & Hennig, S. (2018). RNA structure and cellular applications of fluorescent light-up Aptamers. Angewandte Chemie International Edition, 58(5), 1266–1279. https://doi.org/10.1002/anie.201806482
 
Neubacher, S., & Hennig, S. (2018). RNA structure and cellular applications of fluorescent light-up Aptamers. Angewandte Chemie International Edition, 58(5), 1266–1279. https://doi.org/10.1002/anie.201806482
  
 
Paige, J. S., Wu, K. Y., & Jaffrey, S. R. (2011). RNA mimics of green fluorescent protein. Science, 333(6042), 642–646. https://doi.org/10.1126/science.1207339
 
Paige, J. S., Wu, K. Y., & Jaffrey, S. R. (2011). RNA mimics of green fluorescent protein. Science, 333(6042), 642–646. https://doi.org/10.1126/science.1207339

Latest revision as of 16:10, 10 October 2022


iSpinach-D5-G30-A32 aptamer with LacI repression


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 366
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part works identically to BBa_K4245001, a composite part producing Spinach, and BBa_K4245002, a composite part producing iSpinach; the only difference is the aptamer produced. This disparity allows accurate comparison of the basic aptamer parts.

Source

The part was acquired as synthesized sequences from IDT and Twist Bioscience.

References

Fernandez-Millan, P., Autour, A., Ennifar, E., Westhof, E., & Ryckelynck, M. (2017). Crystal structure and fluorescence properties of the iSpinach aptamer in complex with DFHBI. RNA, 23(12), 1788–1795. https://doi.org/10.1261/rna.063008.117

Neubacher, S., & Hennig, S. (2018). RNA structure and cellular applications of fluorescent light-up Aptamers. Angewandte Chemie International Edition, 58(5), 1266–1279. https://doi.org/10.1002/anie.201806482

Paige, J. S., Wu, K. Y., & Jaffrey, S. R. (2011). RNA mimics of green fluorescent protein. Science, 333(6042), 642–646. https://doi.org/10.1126/science.1207339