Difference between revisions of "Part:BBa K4165206"

 
 
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Fusion protein consists of Tau binding peptide (M4W39) for tau targeting and Coh2 protein [BBa_K4165003] that bind to Docs [BBa_K3396000] to form the protac system.
 
Fusion protein consists of Tau binding peptide (M4W39) for tau targeting and Coh2 protein [BBa_K4165003] that bind to Docs [BBa_K3396000] to form the protac system.
  
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===Usage and Biology===
 
===Usage and Biology===
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DOC - CoH system was used for many targeting biological applications . In our case we introduce a fusion protein with Tau binding peptide (M4W39) for targeting Tau  with different methods so aggregations inhibition can be further tested in vivo using this part.
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<span class='h3bb'>Sequence and Features</span>
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===<span class='h3bb'>Sequence and Features</span>===
 
<partinfo>BBa_K4165206 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4165206 SequenceAndFeatures</partinfo>
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===3D Model Photo and ranking===
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This fused protein was modeled using RosettaFold software and after running our quality assessment code it got a score of 4 out of 6 according to the parameters of the QA code: C-beta deviations, clash score, Molprobity, Ramachandran favored, Ramachandran outliers, Q mean 4, and Q mean 6.
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This protein was excluded due to its low QA score as compared to other models
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<p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/mmm.png" style="margin-left:200px;" alt="" width="500" /></p>
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                      Figure 1. Predicted 3D structure of CoH2-(G4S)3-M4W39 protein Visualized by Pymol.
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Latest revision as of 08:55, 13 October 2022


Coh2 linked with tau binding peptide (M4W39)

Fusion protein consists of Tau binding peptide (M4W39) for tau targeting and Coh2 protein [BBa_K4165003] that bind to Docs [BBa_K3396000] to form the protac system.

Usage and Biology

DOC - CoH system was used for many targeting biological applications . In our case we introduce a fusion protein with Tau binding peptide (M4W39) for targeting Tau with different methods so aggregations inhibition can be further tested in vivo using this part.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

3D Model Photo and ranking

This fused protein was modeled using RosettaFold software and after running our quality assessment code it got a score of 4 out of 6 according to the parameters of the QA code: C-beta deviations, clash score, Molprobity, Ramachandran favored, Ramachandran outliers, Q mean 4, and Q mean 6. This protein was excluded due to its low QA score as compared to other models 

                     Figure 1. Predicted 3D structure of CoH2-(G4S)3-M4W39 protein Visualized by Pymol.