Difference between revisions of "Part:BBa K4239005:Design"
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===Design Notes=== | ===Design Notes=== | ||
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| + | <p><i>fiatluxE</i> did not have any mutation compare to <i>iluxE</i>, because no iGEM restriction site (EcoRI, XbaI, SpeI and PstI) were into the gene. | ||
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| + | <p><i>iluxE</i> did not have any mutation compare to <i>luxE</i>, according to Gregor et al.’s study in 2018.</p> | ||
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===Source=== | ===Source=== | ||
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| + | <p>The source of <i>fiatluxA</i> was the <i>ilux</i> operon available in a pGEX plasmid. An overlap PCR was performed to reconstitute directly <i>fiatluxABE</i> fragments which had been cut by the restriction enzymes.</p> | ||
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===References=== | ===References=== | ||
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Gregor C, Gwosch KC, Sahl SJ, Hell SW. Strongly enhanced bacterial bioluminescence with the ilux operon for single-cell imaging. Proc Natl Acad Sci U S A. 2018 Jan 30;115(5):962-967. doi: 10.1073/pnas.1715946115. Epub 2018 Jan 16. PMID: 29339494; PMCID: PMC5798359. | Gregor C, Gwosch KC, Sahl SJ, Hell SW. Strongly enhanced bacterial bioluminescence with the ilux operon for single-cell imaging. Proc Natl Acad Sci U S A. 2018 Jan 30;115(5):962-967. doi: 10.1073/pnas.1715946115. Epub 2018 Jan 16. PMID: 29339494; PMCID: PMC5798359. | ||
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Latest revision as of 00:20, 12 October 2022
Enhanced luciferase substrate forming unit fiatluxE
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 588
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
fiatluxE did not have any mutation compare to iluxE, because no iGEM restriction site (EcoRI, XbaI, SpeI and PstI) were into the gene.
iluxE did not have any mutation compare to luxE, according to Gregor et al.’s study in 2018.
Source
The source of fiatluxA was the ilux operon available in a pGEX plasmid. An overlap PCR was performed to reconstitute directly fiatluxABE fragments which had been cut by the restriction enzymes.
References
Gregor C, Gwosch KC, Sahl SJ, Hell SW. Strongly enhanced bacterial bioluminescence with the ilux operon for single-cell imaging. Proc Natl Acad Sci U S A. 2018 Jan 30;115(5):962-967. doi: 10.1073/pnas.1715946115. Epub 2018 Jan 16. PMID: 29339494; PMCID: PMC5798359.