Difference between revisions of "Part:BBa K4239002:Design"

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===Design Notes===
 
===Design Notes===
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Every Igem restriction sites (EcoR1, Xba1, Spe1 and Pst1) were removed from the part.
 
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Codon optimization is done for E.coli and D.solani.
 
  
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<p>The Igem restriction site XbaI was removed into the <i>iluxD</i> part, to create <i>fiatluxD</i>. An Adenine was replace by a Guanine. The mutation does not change the protein sequence.</p>
  
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<p><i>iluxD</i> did not have any mutation compare to <i>luxD</i>, according to Gregor et al.’s study in 2018.</p>
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===Source===
 
===Source===
  
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fiatlux is made up with fiatluxA, fiatluxB and fiatluxE
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<p>The source of <i>fiatluxD</i> is synthesis. This part was synthetized from the nucleotidique sequence according to Gregor et al.’s study in 2018.</p>
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<p>This part was synthetized directly with <i>fiatluxC</i> and the promoter BBa_J23117.</p>
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===References===
 
===References===
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Gregor C, Gwosch KC, Sahl SJ, Hell SW. Strongly enhanced bacterial bioluminescence with the ilux operon for single-cell imaging. Proc Natl Acad Sci U S A. 2018 Jan 30;115(5):962-967. doi: 10.1073/pnas.1715946115. Epub 2018 Jan 16. PMID: 29339494; PMCID: PMC5798359.
 
Gregor C, Gwosch KC, Sahl SJ, Hell SW. Strongly enhanced bacterial bioluminescence with the ilux operon for single-cell imaging. Proc Natl Acad Sci U S A. 2018 Jan 30;115(5):962-967. doi: 10.1073/pnas.1715946115. Epub 2018 Jan 16. PMID: 29339494; PMCID: PMC5798359.
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Latest revision as of 00:09, 12 October 2022


Enhanced luciferase subunits fiatluxD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The Igem restriction site XbaI was removed into the iluxD part, to create fiatluxD. An Adenine was replace by a Guanine. The mutation does not change the protein sequence.

iluxD did not have any mutation compare to luxD, according to Gregor et al.’s study in 2018.

Source

The source of fiatluxD is synthesis. This part was synthetized from the nucleotidique sequence according to Gregor et al.’s study in 2018.

This part was synthetized directly with fiatluxC and the promoter BBa_J23117.

References

Gregor C, Gwosch KC, Sahl SJ, Hell SW. Strongly enhanced bacterial bioluminescence with the ilux operon for single-cell imaging. Proc Natl Acad Sci U S A. 2018 Jan 30;115(5):962-967. doi: 10.1073/pnas.1715946115. Epub 2018 Jan 16. PMID: 29339494; PMCID: PMC5798359.