Difference between revisions of "Part:BBa K4385014:Design"
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===Source=== | ===Source=== | ||
− | + | The basic part PbrR comes from Cupriavidus metallidurans strain CH34, other parts all from E. coli genomic DNA. | |
===References=== | ===References=== |
Latest revision as of 15:46, 6 October 2022
Lead constitutive surface display
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 938
Illegal PstI site found at 880 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 1120
Illegal NheI site found at 1143
Illegal SpeI site found at 938
Illegal PstI site found at 880 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1255
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 938
Illegal PstI site found at 880 - 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 938
Illegal PstI site found at 880 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 671
Design Notes
The part mainly consists of the constitutive promoter (BBa_J23100), the lead-inducible regulatory protein PbrR (BBa_K4385000), the outer membrane protein A(BBa_K4385002) and the lipoprotein of E. coli(BBa_K4385001).
Source
The basic part PbrR comes from Cupriavidus metallidurans strain CH34, other parts all from E. coli genomic DNA.