Difference between revisions of "Part:BBa K4204014:Design"
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The toehold switch would be at the 5' end of the mRNA with a corresponding trigger at the 3' end of the mRNA. | The toehold switch would be at the 5' end of the mRNA with a corresponding trigger at the 3' end of the mRNA. | ||
This synthetic protein quality control system is believed to have no limitations regarding its use in other bacteria, and this system can be applied to improve the quality of recombinant protein production and also efficiencies of metabolic pathways. | This synthetic protein quality control system is believed to have no limitations regarding its use in other bacteria, and this system can be applied to improve the quality of recombinant protein production and also efficiencies of metabolic pathways. | ||
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===Source=== | ===Source=== | ||
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===References=== | ===References=== | ||
+ | [1] Yang J, Han YH, Im J, Seo SW. Synthetic protein quality control to enhance full-length translation in bacteria. Nat Chem Biol. 2021 Apr;17(4):421-427. doi: 10.1038/s41589-021-00736-3. Epub 2021 Feb 4. PMID: 33542534. | ||
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+ | [2] Lee, K. O., Kong, M., Kim, I., Bai, J., Cha, S., Kim, B., Ryu, K.-S., Ryu, S., & Suh, J.-Y. (2019). Structural basis for cell-wall recognition by bacteriophage PBC5 endolysin. Structure, 27(9). https://doi.org/10.1016/j.str.2019.07.001 |
Latest revision as of 10:59, 12 October 2022
Quality control system + LysPBC5
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 907
Illegal PstI site found at 31
Illegal PstI site found at 367 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 907
Illegal NheI site found at 1017
Illegal NheI site found at 1099
Illegal PstI site found at 31
Illegal PstI site found at 367 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 907
Illegal BglII site found at 916
Illegal BamHI site found at 1794
Illegal XhoI site found at 2122 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 907
Illegal PstI site found at 31
Illegal PstI site found at 367 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 907
Illegal PstI site found at 31
Illegal PstI site found at 367
Illegal NgoMIV site found at 346
Illegal NgoMIV site found at 1846
Illegal AgeI site found at 496 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The toehold switch would be at the 5' end of the mRNA with a corresponding trigger at the 3' end of the mRNA. This synthetic protein quality control system is believed to have no limitations regarding its use in other bacteria, and this system can be applied to improve the quality of recombinant protein production and also efficiencies of metabolic pathways.
Source
Eukaryotic translation system.
References
[1] Yang J, Han YH, Im J, Seo SW. Synthetic protein quality control to enhance full-length translation in bacteria. Nat Chem Biol. 2021 Apr;17(4):421-427. doi: 10.1038/s41589-021-00736-3. Epub 2021 Feb 4. PMID: 33542534.
[2] Lee, K. O., Kong, M., Kim, I., Bai, J., Cha, S., Kim, B., Ryu, K.-S., Ryu, S., & Suh, J.-Y. (2019). Structural basis for cell-wall recognition by bacteriophage PBC5 endolysin. Structure, 27(9). https://doi.org/10.1016/j.str.2019.07.001