Difference between revisions of "Part:BBa K4204013"

Latest revision as of 06:00, 12 October 2022

This part is a His-tagged LysPBC5, used for extraction and purification of this protein. For detailed information about LysPBC5, see BBa_K4204000

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-==iPTG+sfGFP+quality control system==
+This part is a His-tagged LysPBC5, used for extraction and purification of this protein.
+For detailed information about LysPBC5, see BBa_K4204000
-===Description===
-The protein quality control system (Pro-QC system) is universal that can be applied to all kinds of protein expression. The system contains a toehold switch (BBa_K4204005) at the 5' end of an mRNA and a corresponding trigger (Cis-trigger) (BBa_K4204006)sequence with reverse complementarity to the toehold switch at the 3' end of the mRNA. The switch binds with its complementary sequence and blocks the RBS inside a neck-ring structure; this blocks transcription initiation. When Cis-trigger appears, the switch will bind with the trigger preferentially because the Cis-trigger has a higher affinity with the switch. Thus, the switch and the trigger RNA pairs deformed the neck-ring structure to free the RBS and circularized the mRNA regardless of the gene of interest The exposed RBS enables translation initiation. Thus only the full-length mRNAs with trigger sequence at its 3’end are selectively used as the templates for translation. The sfGFP sequences can be replaced. Thus, other IGEM teams can use the protein quality control system.
 ===Sequence and Features===
 <partinfo>BBa_K4204013 SequenceAndFeatures</partinfo>