Difference between revisions of "Part:BBa K4162029"

Latest revision as of 15:08, 12 October 2022

ribozyme + T7_RBS + BCMO + ribozyme + T7_RBS + ybbO-tdMCP-EGFP

Introduction

Coding sequence of BCMO and gene ybbO separated by hammerhead ribozyme. Gene ybbO is tagged with tdMCP and EGFP.

We use this biobrick to verify the expression ybbO in the presence of BCMO. Please check our measurement page for details.

Figure 1. Fluorescent imaging revealed that IPTG induction increases the expression of both BCMO and ybbO. We observed fluorescence images of Hi-control Rosetta strain (1. Bacteria expressing BCMO-linker-StayGold; 2. Bacteria expressing BCMO and ybbO-tdMCP-GFP) through an Olympus fluorescence microscope. Samples induced with IPTG have stronger signal than controls.

Figure 1. Fluorescent imaging confirm the expression of both BCMO and ybbO. We observed fluorescence images of E. coli (1. Bacteria expressing BCMO-linker-StayGold ; 2. Bacteria expressing BCMO and ybbO-tdMCP-GFP ) through an Olympus fluorescence microscope with a 1.45 NA 150× oil objective. GFP and StayGold were excited by a 488 nm laser. Images were taken by the same settings for comparison.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 2521
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 2521
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 2521
Illegal AgeI site found at 1018
Illegal AgeI site found at 1611
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 2101
Illegal BsaI site found at 2455

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4162029 short</partinfo>
-Coding sequence of BCMO and gene ybbO separated by hammerhead ribozyme. Gene ybbO is tagged with tdMCP and EGFP.
+===Introduction===
+[[File:T--Fudan--logo-Rester-rectangle.jpg|100px|right|2022 Fudan]]
+Coding sequence of BCMO and gene ''ybbO'' separated by hammerhead ribozyme. Gene ''ybbO'' is tagged with tdMCP and EGFP.
+We use this biobrick to verify the expression ybbO in the presence of BCMO. Please check our [https://2022.igem.wiki/fudan/measurement#fluorescence-microscopy-to-confirm-the-successful-expression-of-membrane-proteins measurement] page for details.
+[[File:T--Fudan--micro--m1-IPTG.png|400px|thumb|none|'''Figure 1. Fluorescent imaging revealed that IPTG induction increases the expression of both BCMO and ybbO.''' We observed fluorescence images of Hi-control Rosetta strain (1. Bacteria expressing BCMO-linker-StayGold; 2. Bacteria expressing BCMO and ybbO-tdMCP-GFP) through an Olympus fluorescence microscope. Samples induced with IPTG have stronger signal than controls.]]
+[[File:T--Fudan--micro--m2-BCMO-ybbO.png|400px|thumb|none|'''Figure 1. Fluorescent imaging confirm the expression of both BCMO and ybbO.
+''' We observed fluorescence images of E. coli (1. Bacteria expressing BCMO-linker-StayGold ; 2. Bacteria expressing BCMO and ybbO-tdMCP-GFP ) through an Olympus fluorescence microscope with a 1.45 NA 150× oil objective. GFP and StayGold were excited by a 488 nm laser. Images were taken by the same settings for comparison.]]
 <!-- Add more about the biology of this part here