Difference between revisions of "Part:BBa K4165070"
 
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<partinfo>BBa_K4165070 short</partinfo>
 
<partinfo>BBa_K4165070 short</partinfo>
  
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This Part encodes for the GST tag which serve as an enhancing tag and also serves in purification of expressed proteins in bacterial expression system.
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This Part encodes the GST tag which serve as an enhancing tag and also serves in purification of expressed proteins in bacterial expression system.
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===Usage and Biology===
 
===Usage and Biology===
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Glutathione S-transferase (GST), used for affinity purification when fused to a gene of interest [1]. Glutathione-agarose affinity chromatography provides the quick, delicate, non-denaturing, and very selective purification of glutathione-binding sequence-containing proteins, such as Glutathione S-Transferase (GST), glutathione peroxidase, and glyoxalase. (DocS and Coh are fused with both GST and His tag to determine which one will give more yield and stability)
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Glutathione S-transferase (GST), used for affinity purification when fused to a gene of interest [1]. Glutathione-agarose affinity chromatography provides the quick, delicate, non-denaturing, and very selective purification of glutathione-binding sequence-containing proteins, such as Glutathione S-Transferase (GST), glutathione peroxidase, and glyoxalase.  
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===Source===
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In our case, we also used GST to enhance the stability and expression yield of our protein pair DocS and Coh2, so we fused them once with GST and once with His tag to determine which one will give more yield and stability.
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(pGS-21a) vector, the backbone retrieved from add gene
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===References===
 
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1- Harper, S., & Speicher, D. W. (2011). Purification of proteins fused to glutathione S-tranferase. Methods in molecular biology (Clifton, N.J.), 681, 259. https://doi.org/10.1007/978-1-60761-913-0_14.
 
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<span class='h3bb'>Sequence and Features</span>
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===<span class='h3bb'>Sequence and Features</span>===
 
<partinfo>BBa_K4165070 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4165070 SequenceAndFeatures</partinfo>
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===References===
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1- Harper, S., & Speicher, D. W. (2011). Purification of proteins fused to glutathione S-tranferase. Methods in molecular biology (Clifton, N.J.), 681, 259. https://doi.org/10.1007/978-1-60761-913-0_14.
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Latest revision as of 13:38, 10 October 2022


Glutathione S-Transferase

This Part encodes the GST tag which serve as an enhancing tag and also serves in purification of expressed proteins in bacterial expression system.


Usage and Biology

Glutathione S-transferase (GST), used for affinity purification when fused to a gene of interest [1]. Glutathione-agarose affinity chromatography provides the quick, delicate, non-denaturing, and very selective purification of glutathione-binding sequence-containing proteins, such as Glutathione S-Transferase (GST), glutathione peroxidase, and glyoxalase.

In our case, we also used GST to enhance the stability and expression yield of our protein pair DocS and Coh2, so we fused them once with GST and once with His tag to determine which one will give more yield and stability.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 85


References

1- Harper, S., & Speicher, D. W. (2011). Purification of proteins fused to glutathione S-tranferase. Methods in molecular biology (Clifton, N.J.), 681, 259. https://doi.org/10.1007/978-1-60761-913-0_14.