Difference between revisions of "Part:BBa K4165020"
 
(7 intermediate revisions by 2 users not shown)
Line 3: Line 3:
 
<partinfo>BBa_K4165020 short</partinfo>
 
<partinfo>BBa_K4165020 short</partinfo>
  
−
This part encodes for 6x Histidine tag used for purification and characterization of proteins
+
This part encodes 6x Histidine tag used for purification and characterization of proteins
  
 
===Usage and Biology===
 
===Usage and Biology===
−
His tag is used for purifying or detecting the recombinant protein without a protein-specific antibody or probe (1). All parts of our trim system are fused with his tag for purification.
+
His tag is used for purifying and detecting the recombinant protein without a protein-specific antibody or probe <sup>[1]</sup>. Parts of our systems are fused with his tag for purification and pull-down assay.
 +
 
 +
<!-- -->
 +
===<span class='h3bb'>Sequence and Features</span>===
 +
<partinfo>BBa_K4165020 SequenceAndFeatures</partinfo>
  
−
===Source===
 
−
(pGS-21a) vector, the backbone was retrieved from add gene.
 
  
 
===References===
 
===References===
 
1- Spriestersbach A, Kubicek J, Schäfer F, Block H, Maertens B. Purification of His-Tagged Proteins. Methods Enzymol. 2015;559:1-15. doi: 10.1016/bs.mie.2014.11.003. Epub 2015 May 4. PMID: 26096499.
 
1- Spriestersbach A, Kubicek J, Schäfer F, Block H, Maertens B. Purification of His-Tagged Proteins. Methods Enzymol. 2015;559:1-15. doi: 10.1016/bs.mie.2014.11.003. Epub 2015 May 4. PMID: 26096499.
−
 
−
<!-- -->
 
−
<span class='h3bb'>Sequence and Features</span>
 
−
<partinfo>BBa_K4165020 SequenceAndFeatures</partinfo>
 
  
  

Latest revision as of 13:30, 10 October 2022


6x Histidine tag

This part encodes 6x Histidine tag used for purification and characterization of proteins

Usage and Biology

His tag is used for purifying and detecting the recombinant protein without a protein-specific antibody or probe [1]. Parts of our systems are fused with his tag for purification and pull-down assay.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

1- Spriestersbach A, Kubicek J, Schäfer F, Block H, Maertens B. Purification of His-Tagged Proteins. Methods Enzymol. 2015;559:1-15. doi: 10.1016/bs.mie.2014.11.003. Epub 2015 May 4. PMID: 26096499.