Difference between revisions of "Part:BBa K4321004"
 
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<partinfo>BBa_K4321004 short</partinfo>
 
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mScarlet is a red fluorescent protein (RFP) which was engineered in 2017 and based on a syntheic gene template consisting of mCherry, chromo proteins, and naturally occurring RFPs.  
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mScarlet is a red fluorescent protein (RFP) which was engineered in 2017 and based on a synthetic gene template consisting of mCherry, chromo proteins, and naturally occurring RFPs.  
  
  
 
===Usage and Biology===
 
===Usage and Biology===
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This fluorescent protein carries the highest brightness in the RFP class and a fluorescent life of 3.9 nanoseconds. mScarlet has an absorbance of 569 nanometers and and emission maxima of 594 nanometers.  
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This fluorescent protein carries the highest brightness in the RFP class and a fluorescent life of 3.9 nanoseconds. mScarlet has an absorbance of 569 nanometers and emission maxima of 594 nanometers.  
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<span class='h3bb'>Sequence and Features</span>
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===Sequence and Features===
 
<partinfo>BBa_K4321004 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4321004 SequenceAndFeatures</partinfo>
  
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==References==
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Bindels, D., Haarbosch, L., van Weeren, L. et al. mScarlet: a bright monomeric red fluorescent protein for cellular imaging. Nat Methods 14, 53–56 (2017). https://doi.org/10.1038/nmeth.4074
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mScarlet . SnapGene. (n.d.). Retrieved October 4, 2022, from https://www.snapgene.com/resources/plasmid-files/?set=fluorescent_protein_genes_and_plasmids&amp;plasmid=mScarlet
  
 
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Latest revision as of 21:04, 9 October 2022


mScarlet

mScarlet is a red fluorescent protein (RFP) which was engineered in 2017 and based on a synthetic gene template consisting of mCherry, chromo proteins, and naturally occurring RFPs.


Usage and Biology

This fluorescent protein carries the highest brightness in the RFP class and a fluorescent life of 3.9 nanoseconds. mScarlet has an absorbance of 569 nanometers and emission maxima of 594 nanometers.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 512
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

Bindels, D., Haarbosch, L., van Weeren, L. et al. mScarlet: a bright monomeric red fluorescent protein for cellular imaging. Nat Methods 14, 53–56 (2017). https://doi.org/10.1038/nmeth.4074

mScarlet . SnapGene. (n.d.). Retrieved October 4, 2022, from https://www.snapgene.com/resources/plasmid-files/?set=fluorescent_protein_genes_and_plasmids&plasmid=mScarlet