Difference between revisions of "Part:BBa K4165157"
 
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<partinfo>BBa_K4165157 short</partinfo>
 
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Tau binding peptide targeting the PHF seed of Tau
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A synthetic peptide that is used for targeting misfolded tau protein (BBa_K4165009) as it binds to PHF6 of tau fibrils.
  
 
===Usage and Biology===
 
===Usage and Biology===
  
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MMDP2 peptide sequence is HSDLWRRSFELM, it binds to <sup>275</sup>-VQIINK-<sup>280</sup> (PHF*) and <sup>306</sup>-VQIVYK-<sup>311</sup> (PHF) motifs of tau that are reported to drive tau paired helical filament aggregates. it inhibits formation of PHF6* aggregates, but not PHF and full length tau driven aggregates.
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MMDP2 peptide sequence is HSDLWRRSFELM, it binds to <sup>275</sup>-VQIINK-<sup>280</sup> (PHF*) and <sup>306</sup>-VQIVYK-<sup>311</sup> (PHF) motifs of tau that are reported to drive tau paired helical filament aggregates, it inhibits formation of PHF* aggregates, but not PHF and full-length tau driven aggregates.
  
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<span class='h3bb'> <p style=" font-weight: bold; font-size:17px;"> Sequence and Features</p> </span>
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4165157 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4165157 SequenceAndFeatures</partinfo>
  
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<p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/mmdp6.png" style="margin-left:200px;" alt="" width="300" /></p>
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<p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/mmdp6.png" style="margin-left:300px;" alt="" width="300" /></p>
 
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<p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/mmdp6-qa.png" style="margin-left:75px;" alt="" width="800" /></p>
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<p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/mmdp6-qa.png" style="margin-left:50px;" alt="" width="800" /></p>
 
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===References===
 
===References===

Latest revision as of 14:58, 11 October 2022


MMDP6 Peptide

A synthetic peptide that is used for targeting misfolded tau protein (BBa_K4165009) as it binds to PHF6 of tau fibrils.

Usage and Biology

MMDP2 peptide sequence is HSDLWRRSFELM, it binds to 275-VQIINK-280 (PHF*) and 306-VQIVYK-311 (PHF) motifs of tau that are reported to drive tau paired helical filament aggregates, it inhibits formation of PHF* aggregates, but not PHF and full-length tau driven aggregates.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Dry Lab

Modeling

MMDP6 is modeled by AlphaFold2 and Apptest, best model obtained from AlphaFold2 


                            Figure 1.: Predicted 3D structure of Synthetic peptide MMDP6.


Table 1: Quality assessment parameters of MMDP6 model.

References

1. Malhis, M., Kaniyappan, S., Aillaud, I., Chandupatla, R. R., Ramirez, L. M., Zweckstetter, M., ... & Funke, S. A. (2021). Potent Tau Aggregation Inhibitor D‐Peptides Selected against Tau‐Repeat 2 Using Mirror Image Phage Display. ChemBioChem, 22(21), 3049-3059.