Difference between revisions of "Part:BBa K4165152"
 
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<partinfo>BBa_K4165152 short</partinfo>
 
<partinfo>BBa_K4165152 short</partinfo>
  
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Tau binding peptide targeting the PHF seed of Tau
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A synthetic peptide that is used for targeting misfolded tau protein (BBa_K4165009) as it binds to PHF6 of tau fibrils.
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===Usage and Biology===
 
===Usage and Biology===
  
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ISAD1 peptide with amino acid sequence of SVFKLSLTDAAS. It acts as an inhibitor for wild type full length tau aggregation.   
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ISAD1 peptide with amino acid sequence of SVFKLSLTDAAS. It acts as an inhibitor for wild type full length tau aggregations.   
  
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<span class='h3bb'> <p style=" font-weight: bold; font-size:17px;"> Sequence and Features</p> </span>
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4165152 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4165152 SequenceAndFeatures</partinfo>
  
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<p style=" font-weight: bold; font-size:14px;"> Modeling </p>
 
<p style=" font-weight: bold; font-size:14px;"> Modeling </p>
 
   
 
   
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ISAD1 is modeled by AlphaFold2, Apptest, ITASSER, best model obtained from Apptest.
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ISAD1 was modeled by AlphaFold2, Apptest, ITASSER, and the best model was obtained from Apptest ranking 2 out of 6.
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<p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/isad1.png" style="margin-left:200px;" alt="" width="300" /></p>
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<p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/isad1.png" style="margin-left:300px;" alt="" width="300" /></p>
 
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<p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/isal1-qa.png" style="margin-left:75px;" alt="" width="800" /></p>
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<p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/isal1-qa.png" style="margin-left:50px;" alt="" width="800" /></p>
 
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===References===
 
===References===

Latest revision as of 14:46, 11 October 2022


ISAD1 Peptide

A synthetic peptide that is used for targeting misfolded tau protein (BBa_K4165009) as it binds to PHF6 of tau fibrils.

Usage and Biology

ISAD1 peptide with amino acid sequence of SVFKLSLTDAAS. It acts as an inhibitor for wild type full length tau aggregations.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Dry Lab

Modeling

ISAD1 was modeled by AlphaFold2, Apptest, ITASSER, and the best model was obtained from Apptest ranking 2 out of 6. 


                            Figure 1.: Predicted 3D structure of Synthetic peptide ISAD1.


Table 1: Quality assessment parameters of ISAD1 model.

References

1. Aillaud, I., Kaniyappan, S., Chandupatla, R. R., Ramirez, L. M., Alkhashrom, S., Eichler, J., ... & Funke, S. A. (2022). A novel D-amino acid peptide with therapeutic potential (ISAD1) inhibits aggregation of neurotoxic disease-relevant mutant Tau and prevents Tau toxicity in vitro. Alzheimer's research & therapy, 14(1), 1-19.