Difference between revisions of "Part:BBa K4121022:Design"

 
 
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===Design Notes===
 
===Design Notes===
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Before using this part, give priority to whether it has the enzyme cleavage site required, if so, consider site-directed mutagenesis.
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The N-terminal interception gene tHMG1 of the HMG-CoA reductase gene encoding HMG1 in the mevalonate pathway. HMG1 can catalyze the conversion of HMG-CoA to mevalonate.
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Latest revision as of 11:29, 7 October 2022


The mevalonate kinase gene ERG12


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 734
    Illegal XbaI site found at 816
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 734
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 734
    Illegal BglII site found at 753
    Illegal BamHI site found at 297
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 734
    Illegal XbaI site found at 816
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 734
    Illegal XbaI site found at 816
    Illegal AgeI site found at 1048
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The N-terminal interception gene tHMG1 of the HMG-CoA reductase gene encoding HMG1 in the mevalonate pathway. HMG1 can catalyze the conversion of HMG-CoA to mevalonate.


Source

National Center for Biotechnology Information (NCBI)

References