Difference between revisions of "Part:BBa K4268000:Experience"

(→‎Applications of BBa_K4268000)
 
(8 intermediate revisions by the same user not shown)
Line 1: Line 1:
  
 
__NOTOC__
 
__NOTOC__
−
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
+
The part was received, resuspended, and cloned into the level 0 Golden Gate vector, PSB1C00. The ligation was then transformed into DH5&alpha; cells. After overnight growth, white colonies (indicative of a replacement of the RFP reporter cassette) were subjected to screening for the correct insert using colony PCR with the VF/VR primer pair.
−
how you used this part and how it worked out.
+
  
−
===Applications of BBa_K4268000===
 
−
The part was received, resuspended, and cloned into the level 0 Golden Gate vector, PSB1C00. The ligation was then transformed into DH5&alpha cells.  After overnight growth, white colonies (indicative of a replacement of the RFP reporter cassette) were subjected to screening for the correct insert using colony PCR with the VF/VR primer pair.
 
  
 +
[[File: T-suny-oneonta-t7-head tail connector colony PCR figure.png|500px|thumb|center|Figure 1: Colony PCR of five colonies (clones) suspected of containing the Head-tail connector insert. The insert length is 1573bp and the predicted size of the PCR product when using VF/VR primers is 1878 bp.]]
  
−
[[File: T-suny-oneonta-t7-head-tail connector colony PCR figure.png|200px|border|center|Figure 1: Colony PCR of five colonies (clones) suspected of coating the insert Head-tail connector insert. The insert length is 1573bp and the predicted size of the PCR product using VF/VR primers is 1739 bp.]]
+
The gel indicates that all five colonies are likely to contain the correct insert and thus, the Head-Tail Connector was successfully cloned into a Level 0 Golden Gate Assembly basic part.
  
−
The gel indicates that all five colonies are likely to contain the correct insert and thus, the Head-Tail Connector was successfully cloned into a Level O Golden Gate Assembly basic part.
+
 
 +
===Applications of BBa_K4268000===
  
 
===User Reviews===
 
===User Reviews===
Line 21: Line 20:
 
<I>Username</I>
 
<I>Username</I>
 
|width='60%' valign='top'|
 
|width='60%' valign='top'|
−
Enter the review inofrmation here.
+
Enter the review information here.
 
|};
 
|};
 
<!-- End of the user review template -->
 
<!-- End of the user review template -->
 
<!-- DON'T DELETE --><partinfo>BBa_K4268000 EndReviews</partinfo>
 
<!-- DON'T DELETE --><partinfo>BBa_K4268000 EndReviews</partinfo>

Latest revision as of 18:21, 8 October 2022


The part was received, resuspended, and cloned into the level 0 Golden Gate vector, PSB1C00. The ligation was then transformed into DH5α cells. After overnight growth, white colonies (indicative of a replacement of the RFP reporter cassette) were subjected to screening for the correct insert using colony PCR with the VF/VR primer pair.


Figure 1: Colony PCR of five colonies (clones) suspected of containing the Head-tail connector insert. The insert length is 1573bp and the predicted size of the PCR product when using VF/VR primers is 1878 bp.

The gel indicates that all five colonies are likely to contain the correct insert and thus, the Head-Tail Connector was successfully cloned into a Level 0 Golden Gate Assembly basic part.


Applications of BBa_K4268000

User Reviews

UNIQ81d3277e55fdc0f8-partinfo-00000000-QINU UNIQ81d3277e55fdc0f8-partinfo-00000001-QINU