Difference between revisions of "Part:BBa K4165001:Design"
 
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===Design Notes===
 
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The original part on NUDT 2020 parts page had an extra nucleotide at the end so we removed it in order to avoid frameshift. Also, the whole sequence was optimized according to E.Coli BL21
 
  
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===Dry Lab Characterization===
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===Source===
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<p style=" font-weight: bold; font-size:20px;"> Optimization </p>
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Original Trim21 is expressed in humans. We acquired the sequence from iGEM team NUDT_2020 (BBa_K3396007)
  
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===Design Notes===
  
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===Source===
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An extra nucleotide was removed from the original part sequence on NUDT's part page, and the sequence was codon optimized for expression in E. coli BL21.
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original trim is expressed in human and we acquired the sequence from NUDT iGEM team 2020 BBa_K3396007
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===References===
 
===References===

Latest revision as of 12:34, 9 October 2022


Truncated tripartite motif-containing 21 (TRIM21)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 514


Source

Original Trim21 is expressed in humans. We acquired the sequence from iGEM team NUDT_2020 (BBa_K3396007)

Design Notes

An extra nucleotide was removed from the original part sequence on NUDT's part page, and the sequence was codon optimized for expression in E. coli BL21.

References

1. Clift, D., McEwan, W. A., Labzin, L. I., Konieczny, V., Mogessie, B., James, L. C., & Schuh, M. (2017). A Method for the Acute and Rapid Degradation of Endogenous Proteins. Cell, 171(7), 1692-1706.e18. https://doi.org/10.1016/j.cell.2017.10.033

2. D.L. Mallery, W.A. McEwan, S.R. Bidgood, G.J. Towers, C.M. Johnson, L.C. James Antibodies mediate intracellular immunity through tripartite motif-containing 21 (TRIM21) Proc. Natl. Acad. Sci. USA, 107 (2010), pp. 19985-19990

3. Kleiger, G., & Mayor, T. (2014). Perilous journey: a tour of the ubiquitin-proteasome system. Trends in cell biology, 24(6), 352. https://doi.org/10.1016/j.tcb.2013.12.003

4. L.C. James, A.H. Keeble, Z. Khan, D.A. Rhodes, J. Trowsdale Structural basis for PRYSPRY-mediated tripartite motif (TRIM) protein function Proc. Natl. Acad. Sci. USA, 104 (2007), pp. 6200-

5. Zhang, Y., Li, L., Munir, M., & Qiu, H. (2018). RING-Domain E3 Ligase-Mediated Host–Virus Interactions: Orchestrating Immune Responses by the Host and Antagonizing Immune Defense by Viruses. Frontiers in Immunology. https://doi.org/10.3389/fimmu.2018.01083